{"created":"2023-05-15T14:38:17.652585+00:00","id":49415,"links":{},"metadata":{"_buckets":{"deposit":"f9c9aed4-a6f6-4b77-b4d1-01a3888850ac"},"_deposit":{"created_by":1,"id":"49415","owners":[1],"pid":{"revision_id":0,"type":"depid","value":"49415"},"status":"published"},"_oai":{"id":"oai:repo.qst.go.jp:00049415","sets":["1"]},"author_link":["499105","499108","499107","499113","499114","499112","499111","499109","499106","499110"],"item_8_biblio_info_7":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2018-05","bibliographicIssueDateType":"Issued"},"bibliographicPageEnd":"22","bibliographicPageStart":"15","bibliographicVolumeNumber":"117","bibliographic_titles":[{"bibliographic_title":"Enzyme and Microbial Technology"}]}]},"item_8_description_5":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"The Ef-Man gene was determined to consist of 1131 bp and encode a 377 amino acid protein. The amino acid sequence showed similarity with the endo-1,4-beta-mannanases of Daphnia pulex (62%), Cryptopygus antarcticus (64%), Crassostrea gigas (61%),  Mytilus edulis (60%), and Aplisia kurodai (58%). The gene encoding mature Ef-Man was expressed in Pichia pastoris (GS115 strain). Based on SDS-PAGE analysis, the molecular mass of the purified recombinant Ef-Man (rEf-Man) was estimated to be 39.5 kDa. All catalytically important residues of endo-1,4-beta-mannanases in the glycoside hydrolase (GH) family 5 were conserved in Ef-Man. The optimal temperature for rEf-Man was identified as 60°C. HPLC and HPAEC analyses suggest that Ef-Man requires at least six subsites for efficient hydrolysis and is capable of performing transglycosylation reactions. The overall structure of rEf-Man is similar to those of GH5 family proteins, and tertiary structures around the active site are conserved among endo-1,4-beta-mannanase families. X-ray crystallographic analysis supports the hydrolysis and transglycosylation reaction mechanism determined by HPLC and HPAEC analyses. ","subitem_description_type":"Abstract"}]},"item_8_relation_14":{"attribute_name":"DOI","attribute_value_mlt":[{"subitem_relation_type_id":{"subitem_relation_type_id_text":"10.1016/j.enzmictec.2018.05.014","subitem_relation_type_select":"DOI"}}]},"item_8_relation_17":{"attribute_name":"関連サイト","attribute_value_mlt":[{"subitem_relation_name":[{"subitem_relation_name_text":"https://www.sciencedirect.com/science/article/pii/S0141022918301984?via%3Dihub"}],"subitem_relation_type_id":{"subitem_relation_type_id_text":"https://www.sciencedirect.com/science/article/pii/S0141022918301984?via%3Dihub","subitem_relation_type_select":"URI"}}]},"item_8_source_id_9":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"0141-0229","subitem_source_identifier_type":"ISSN"}]},"item_access_right":{"attribute_name":"アクセス権","attribute_value_mlt":[{"subitem_access_right":"metadata only access","subitem_access_right_uri":"http://purl.org/coar/access_right/c_14cb"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"上田, 光宏"}],"nameIdentifiers":[{"nameIdentifier":"499105","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"平野, 優"}],"nameIdentifiers":[{"nameIdentifier":"499106","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"福原, 宏章"}],"nameIdentifiers":[{"nameIdentifier":"499107","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"中, 裕規"}],"nameIdentifiers":[{"nameIdentifier":"499108","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"中澤, 昌美"}],"nameIdentifiers":[{"nameIdentifier":"499109","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"阪本, 龍司"}],"nameIdentifiers":[{"nameIdentifier":"499110","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Ogata, Yoshiyuki"}],"nameIdentifiers":[{"nameIdentifier":"499111","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"玉田, 太郎"}],"nameIdentifiers":[{"nameIdentifier":"499112","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"平野 優","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"499113","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"玉田 太郎","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"499114","nameIdentifierScheme":"WEKO"}]}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"Gene cloning, expression, and X-ray crystallographic analysis of a β-mannanase from Eisenia fetida","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Gene cloning, expression, and X-ray crystallographic analysis of a β-mannanase from Eisenia fetida"}]},"item_type_id":"8","owner":"1","path":["1"],"pubdate":{"attribute_name":"公開日","attribute_value":"2019-01-09"},"publish_date":"2019-01-09","publish_status":"0","recid":"49415","relation_version_is_last":true,"title":["Gene cloning, expression, and X-ray crystallographic analysis of a β-mannanase from Eisenia fetida"],"weko_creator_id":"1","weko_shared_id":-1},"updated":"2023-05-15T23:19:54.343384+00:00"}