{"created":"2023-05-15T14:38:14.071847+00:00","id":49339,"links":{},"metadata":{"_buckets":{"deposit":"f6683cda-ee7f-407b-9c30-b1f99cbe683a"},"_deposit":{"created_by":1,"id":"49339","owners":[1],"pid":{"revision_id":0,"type":"depid","value":"49339"},"status":"published"},"_oai":{"id":"oai:repo.qst.go.jp:00049339","sets":["1"]},"author_link":["727262","727263","727264","727261"],"item_8_biblio_info_7":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2018-11","bibliographicIssueDateType":"Issued"},"bibliographic_titles":[{"bibliographic_title":"IntechOpen"}]}]},"item_8_description_5":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"The clustered regulatory interspaced short palindromic repeats (CRISPR)/Cas9 method is a powerful tool for genome editing, by introducing a DNA double-strand break (DSB) at the specific site. The gene knock-out can be achieved by the deletion or insertion at the CRISPR/Cas9-mediated DSB site by error-prone nonhomologous end joining repair in targeted cells. However, the gene knock-in is still difficult as compared to the knock-out, because of the low efficiency of homology directed repair with donor DNA in cells. Therefore, to efficiently select the knock-in cells, we developed a complicated donor DNA plasmid containing an antibiotic-resistance gene, in addition to the knock-in sequence and the two homology arms. MultiSite Gateway technology is a useful tool for constructing this complicated plasmid. We describe the MultiSite Gateway technology and provide an overview of the DSB repair pathways to clarify the knock-out and knockin methods by the CRISPR/Cas9 system.","subitem_description_type":"Abstract"}]},"item_8_relation_14":{"attribute_name":"DOI","attribute_value_mlt":[{"subitem_relation_type_id":{"subitem_relation_type_id_text":"10.5772/intechopen.80775","subitem_relation_type_select":"DOI"}}]},"item_access_right":{"attribute_name":"アクセス権","attribute_value_mlt":[{"subitem_access_right":"metadata only access","subitem_access_right_uri":"http://purl.org/coar/access_right/c_14cb"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"安田, 武嗣"}],"nameIdentifiers":[{"nameIdentifier":"727261","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"田嶋, 克史"}],"nameIdentifiers":[{"nameIdentifier":"727262","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"安田 武嗣","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"727263","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"田嶋 克史","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"727264","nameIdentifierScheme":"WEKO"}]}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"MultiSite Gateway Technology Is Useful for Donor DNA Plasmid Construction in CRISPR/Cas9-Mediated Knock- In System","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"MultiSite Gateway Technology Is Useful for Donor DNA Plasmid Construction in CRISPR/Cas9-Mediated Knock- In System"}]},"item_type_id":"8","owner":"1","path":["1"],"pubdate":{"attribute_name":"公開日","attribute_value":"2019-02-28"},"publish_date":"2019-02-28","publish_status":"0","recid":"49339","relation_version_is_last":true,"title":["MultiSite Gateway Technology Is Useful for Donor DNA Plasmid Construction in CRISPR/Cas9-Mediated Knock- In System"],"weko_creator_id":"1","weko_shared_id":-1},"updated":"2023-05-16T08:00:55.621358+00:00"}