{"created":"2023-05-15T14:38:14.027029+00:00","id":49338,"links":{},"metadata":{"_buckets":{"deposit":"57debf08-0472-4f8b-b583-6a2ba6ea319d"},"_deposit":{"created_by":1,"id":"49338","owners":[1],"pid":{"revision_id":0,"type":"depid","value":"49338"},"status":"published"},"_oai":{"id":"oai:repo.qst.go.jp:00049338","sets":["1"]},"author_link":["872540","872541","872545","872539","872544","872543","872537","872542","872538","872546"],"item_8_biblio_info_7":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2018-10","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"1","bibliographicPageEnd":"125","bibliographicPageStart":"116","bibliographicVolumeNumber":"40","bibliographic_titles":[{"bibliographic_title":"Journal of Cerebral Blood Flow & Metabolism"}]}]},"item_8_description_5":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"Accumulation of detrimental glutathione-conjugated metabolites in the brain potentially causes neurological disorders, and must therefore be exported from the brain. However, in vivo mechanisms of glutathione-conjugates efflux from the brain remain unknown. We investigated the involvement of transporters in glutathione-conjugates efflux using 6-bromo-7-[11C]methylpurine ([11C]1), which enters the brain and is converted into its glutathione conjugate, S-(7-[11C]methylpurin-6-yl)glutathione ([11C]2). In mice of control and knockout of P-glycoprotein/breast cancer resistance protein and multidrug resistance-associated protein 2 ([Mrp2]−/−), [11C]2 formed in the brain was rapidly cleared, with no significant difference in efflux rate. In contrast, [11C]2 formed in the brain of Mrp1−/− mice was slowly cleared, whereas [11C]2 microinjected into the brain of control and Mrp1−/− mice was 75% cleared within 60 min, with no significant difference in efflux rate. These suggest that Mrp1 contributes to [11C]2 efflux across cell membranes, but not BBB. Efflux rate of [11C]2 formed in the brain was significantly lower in Mrp4−/− and organic anion transporter 3 (Oat3)−/− mice compared with control mice. In conclusion, Mrp1, Oat3, and Mrp4 mediate [11C]2 efflux from the brain. Mrp1 may contribute to [11C]2 efflux from brain parenchymal cells, while extracellular [11C]2 is likely cleared across the BBB, partly by Oat3 and Mrp4.","subitem_description_type":"Abstract"}]},"item_8_publisher_8":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"SAGE Publications"}]},"item_8_relation_14":{"attribute_name":"DOI","attribute_value_mlt":[{"subitem_relation_type_id":{"subitem_relation_type_id_text":"10.1177/0271678X18808399","subitem_relation_type_select":"DOI"}}]},"item_8_source_id_9":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"0271-678X","subitem_source_identifier_type":"ISSN"}]},"item_access_right":{"attribute_name":"アクセス権","attribute_value_mlt":[{"subitem_access_right":"metadata only access","subitem_access_right_uri":"http://purl.org/coar/access_right/c_14cb"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Okamura, Toshimitsu"}],"nameIdentifiers":[{"nameIdentifier":"872537","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Okada, Maki"}],"nameIdentifiers":[{"nameIdentifier":"872538","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Kikuchi, Tatsuya"}],"nameIdentifiers":[{"nameIdentifier":"872539","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Wakizaka, Hidekatsu"}],"nameIdentifiers":[{"nameIdentifier":"872540","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Ming-Rong, Zhang"}],"nameIdentifiers":[{"nameIdentifier":"872541","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Okamura, Toshimitsu","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"872542","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Okada, Maki","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"872543","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Kikuchi, Tatsuya","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"872544","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Wakizaka, Hidekatsu","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"872545","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Ming-Rong, Zhang","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"872546","nameIdentifierScheme":"WEKO"}]}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"Mechanisms of glutathione-conjugate efflux from the brain into blood: Involvement of multiple transporters in the course ","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Mechanisms of glutathione-conjugate efflux from the brain into blood: Involvement of multiple transporters in the course "}]},"item_type_id":"8","owner":"1","path":["1"],"pubdate":{"attribute_name":"公開日","attribute_value":"2018-12-19"},"publish_date":"2018-12-19","publish_status":"0","recid":"49338","relation_version_is_last":true,"title":["Mechanisms of glutathione-conjugate efflux from the brain into blood: Involvement of multiple transporters in the course "],"weko_creator_id":"1","weko_shared_id":-1},"updated":"2023-05-15T21:50:27.678623+00:00"}