@article{oai:repo.qst.go.jp:00049325,
 author = {Onishi, Masahiro and Okonogi, Noriyuki and Oike, Takahiro and Yoshimoto, Yuya and Sato, Hiro and Suzuki, Yoshiyuki and Kamada, Tadashi and Nakano, Takashi and Onishi, Masahiro and Okonogi, Noriyuki and Yoshimoto, Yuya and Sato, Hiroaki and Suzuki, Yoshiyuki and Kamada, Tadashi and Nakano, Takashi},
 issue = {5},
 journal = {Journal of radiation research},
 month = {Jun},
 note = {Anti-tumor immunity modulates the local effects of radiation therapy. High mobility group box 1 (HMGB1) plays a pivotal role in activating antigen-specific T-cell responses. Here, we examined the relationship between linear energy transfer (LET) and HMGB1 release. We assessed the proportions of KYSE-70, HeLa and SiHa cells surviving after carbon-ion (C-ion) beam irradiation with different LET values, using a clonogenic assay. The D10, the dose at which 10% of cells survived, was calculated using a linear-quadratic model. HMGB1 levels in the culture supernatants of C-ion beam-irradiated tumor cells were assessed by enzyme-linked immunosorbent assay. The D10 doses for 13 keV/μm of C-ion irradiation in KYSE-70, HeLa and SiHa cells were 2.8, 3.9 and 4.1 Gy, respectively, whereas those for 70 keV/μm C-ion irradiation were 1.4, 1.9 and 2.3 Gy, respectively. We found that 70 keV/μm of C-ion irradiation significantly increased HMGB1 levels in the culture supernatants of all cell lines 72 h after irradiation compared with non-irradiated controls. Furthermore, 70 keV/μm of C-ion irradiation significantly increased HMGB1 levels in the culture supernatants of all cell lines 72 h after irradiation compared with 13 keV/μm. The results suggest that HMGB1 release from several cancer cell lines increases with increased LET.},
 pages = {541--546},
 title = {High linear energy transfer carbon-ion irradiation increases the release of the immune mediator high mobility group box 1 from human cancer cells.},
 volume = {59},
 year = {2018}
}