{"created":"2023-05-15T14:38:10.239160+00:00","id":49252,"links":{},"metadata":{"_buckets":{"deposit":"56500a66-6c69-40e8-bd2f-8442db1cc109"},"_deposit":{"created_by":1,"id":"49252","owners":[1],"pid":{"revision_id":0,"type":"depid","value":"49252"},"status":"published"},"_oai":{"id":"oai:repo.qst.go.jp:00049252","sets":["1"]},"author_link":["746078","746080","746075","746082","746076","746081","746079","746077"],"item_8_biblio_info_7":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2018-10","bibliographicIssueDateType":"Issued"},"bibliographicPageEnd":"20","bibliographicPageStart":"15","bibliographicVolumeNumber":"67","bibliographic_titles":[{"bibliographic_title":"Nuclear Medicine and Biology"}]}]},"item_8_description_5":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"Introduction: Significant discrepancies between in vitro and in vivo binding of themuscarinic receptor ligand – 3Hlabeled\nQuinuclidinyl Benzilate (QNB) – have been well documented. Discernable in vivo cerebellar [3H]QNB\nbinding has been observed in mouse brain, despite the maximum number of binding sites (Bmax) being low. In\norder to understand this unique in vivo binding phenomenon, the binding of two muscarinic receptor ligands\n– [3H]QNB and N-[11C]methylpiperidyl Benzilate ([11C]NMPB) – were compared in vivo and in vitro in 3- and\n8-week-old mice.\nMethod: In vitro binding parameters of [3H]QNB were determined using brain homogenates. The time course of\nradioactivity concentration (TACs) in the cerebral cortex and cerebellum was measured following injection of\n[3H]QNB and [11C]NMPB with or without 3 mg/kg of carrier QNB in 3- and 8 week old mice using a dual tracer\nadministration technique. A graphical method was employed for the quantitative analysis of in vivo binding of\nthese radioligands.\nResults: In vitro, the available number of binding sites for cerebral cortical muscarinic receptors increased by 17%\nduring the developmental period studied. Paradoxically, in vivo, we observed a decrease of [3H]QNB binding in\nthe cerebral cortex, while [11C]NMPB binding was markedly increased. In vivo saturation analysis of [3H]QNB\nin 3-week-old mice revealed an apparent positive cooperativity of binding in the cerebral cortex.\nConclusions: Our results support the hypothesis that microenvironmental factors proximal to muscarinic receptors\ncause a local decrease in the cortical free-ligand concentration of [3H]QNB and that this ‘ligand barrier’ is\nmodulated during brain development.\nAdvances in knowledge: The present study demonstrates that the combined use of radiolabeled QNB and NMPB\nhas the potential to reveal the important effects of receptor microenvironmental factors on receptor function\nin the living brain.","subitem_description_type":"Abstract"}]},"item_8_publisher_8":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"Elsevier"}]},"item_8_relation_14":{"attribute_name":"DOI","attribute_value_mlt":[{"subitem_relation_type_id":{"subitem_relation_type_id_text":"10.1016/i.nucmedbio.2018.10.004","subitem_relation_type_select":"DOI"}}]},"item_8_source_id_9":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"0969-8051","subitem_source_identifier_type":"ISSN"}]},"item_access_right":{"attribute_name":"アクセス権","attribute_value_mlt":[{"subitem_access_right":"metadata only access","subitem_access_right_uri":"http://purl.org/coar/access_right/c_14cb"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Inoue, Osamu"}],"nameIdentifiers":[{"nameIdentifier":"746075","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Sato, Toshiyuki"}],"nameIdentifiers":[{"nameIdentifier":"746076","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Kobayashi, Kaoru"}],"nameIdentifiers":[{"nameIdentifier":"746077","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Gee, Antony"}],"nameIdentifiers":[{"nameIdentifier":"746078","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Shukuri, Miho"}],"nameIdentifiers":[{"nameIdentifier":"746079","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Ming-Rong, Zhang"}],"nameIdentifiers":[{"nameIdentifier":"746080","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Inoue, Osamu","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"746081","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Ming-Rong, Zhang","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"746082","nameIdentifierScheme":"WEKO"}]}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"Unexpected decrease in in vivo binding of [3H]QNB in the mouse cerebral cortex in the developing brain - A comparison with [11C]NMPB","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Unexpected decrease in in vivo binding of [3H]QNB in the mouse cerebral cortex in the developing brain - A comparison with [11C]NMPB"}]},"item_type_id":"8","owner":"1","path":["1"],"pubdate":{"attribute_name":"公開日","attribute_value":"2018-11-15"},"publish_date":"2018-11-15","publish_status":"0","recid":"49252","relation_version_is_last":true,"title":["Unexpected decrease in in vivo binding of [3H]QNB in the mouse cerebral cortex in the developing brain - A comparison with [11C]NMPB"],"weko_creator_id":"1","weko_shared_id":-1},"updated":"2023-05-16T01:15:22.458926+00:00"}