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Modulating the splicing activitiy of Tetrahymena ribozyme via RNA self-assembly
https://repo.qst.go.jp/records/49228
https://repo.qst.go.jp/records/4922802379f8a-788e-44a0-88a3-c9a9787c45ea
| Item type | 学術雑誌論文 / Journal Article(1) | |||||
|---|---|---|---|---|---|---|
| 公開日 | 2018-10-31 | |||||
| タイトル | ||||||
| タイトル | Modulating the splicing activitiy of Tetrahymena ribozyme via RNA self-assembly | |||||
| 言語 | ||||||
| 言語 | eng | |||||
| 資源タイプ | ||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
| 資源タイプ | journal article | |||||
| アクセス権 | ||||||
| アクセス権 | metadata only access | |||||
| アクセス権URI | http://purl.org/coar/access_right/c_14cb | |||||
| 著者 |
Hasegawa, Sumitaka
× Hasegawa, Sumitaka× et.al× 長谷川 純崇 |
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| 抄録 | ||||||
| 内容記述タイプ | Abstract | |||||
| 内容記述 | The internal guiding sequence(IGS)is normally located at the 5' end of trans-splicing ribozymes that are derived from the Tetrahymena group I intron, and is required for the recognition of substrate RNAs and for trans-splicing reactions. Here, we separated the Tetrahymena group I intron at the L2 loop to produce two fragments: the IGS-containing substrate, and the IGS-lacking ribozyme. We show here that two fragments can complex not through the IGS interaction but under the guidance of appended interacting nucleotides, and perform trans-splicing. The splicing reactions took place both in vitro and in mammalianc ells, and the spliced mRNA product from the self-assembled ribozyme complex can be translated into functional proteins in vivo. The splicing efficiency was dependent on the length of appending nucleotides. | |||||
| 書誌情報 |
FEBS Letters 巻 580, 号 6, p. 1592-1596, 発行日 2006-03 |
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| ISSN | ||||||
| 収録物識別子タイプ | ISSN | |||||
| 収録物識別子 | 0014-5793 | |||||
