{"created":"2023-05-15T14:38:09.109104+00:00","id":49226,"links":{},"metadata":{"_buckets":{"deposit":"4730cda3-d31c-4358-ba8d-4c5c3b58deb5"},"_deposit":{"created_by":1,"id":"49226","owners":[1],"pid":{"revision_id":0,"type":"depid","value":"49226"},"status":"published"},"_oai":{"id":"oai:repo.qst.go.jp:00049226","sets":["1"]},"author_link":["496691","496689","496688","496687","496690"],"item_8_biblio_info_7":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2008-10","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"16","bibliographicPageEnd":"2691","bibliographicPageStart":"2682","bibliographicVolumeNumber":"9","bibliographic_titles":[{"bibliographic_title":"Chembiochem"}]}]},"item_8_description_5":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"Noninvasive imaging of specific mRNAs in living subjects promises numerous biological and medical applications. Common strategies use fluorescently or radioactively labelled antisense probes to detect target mRNAs through a hybridization mechanism, but have met with limited success in living animals. Here\nwe present a novel molecular imaging approach based on the group I intron of Tetrahymena thermophila for imaging mRNA molecules in vivo. Engineered trans-splicing ribozyme reporters contain three domains, each of which is designed for targeting, splicing, and reporting. They can transduce the target mRNA into a reporter mRNA, leading to the production of reporter enzymes that can be noninvasively imaged in vivo. We have demonstrated this ribozyme-mediated RNA imaging method for imaging a mutant p53 mRNA both in single cells and noninvasively in living mice. After optimization, the ribozyme reporter increases contrast for the transiently expressed target by 180-fold, and by ten-fold for the stably expressed target. siRNA-mediated specific gene silencingof p53 expression has been successfully imaged in real time in vivo. This newribozyme-ba sed RNA reporter system should open up newavenues for in vivo RNA imaging and direct imaging of siRNA inhibition.","subitem_description_type":"Abstract"}]},"item_8_relation_14":{"attribute_name":"DOI","attribute_value_mlt":[{"subitem_relation_type_id":{"subitem_relation_type_id_text":"10.1002/cbic.200800370","subitem_relation_type_select":"DOI"}}]},"item_8_source_id_9":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"1439-4227","subitem_source_identifier_type":"ISSN"}]},"item_access_right":{"attribute_name":"アクセス権","attribute_value_mlt":[{"subitem_access_right":"metadata only access","subitem_access_right_uri":"http://purl.org/coar/access_right/c_14cb"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"So, Min-Kyung"}],"nameIdentifiers":[{"nameIdentifier":"496687","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Gowrishankar, Gayatri"}],"nameIdentifiers":[{"nameIdentifier":"496688","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Hasegawa, Sumitaka"}],"nameIdentifiers":[{"nameIdentifier":"496689","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"et.al"}],"nameIdentifiers":[{"nameIdentifier":"496690","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"長谷川 純崇","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"496691","nameIdentifierScheme":"WEKO"}]}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"Imaging Target mRNA and siRNA-Mediated Gene Silencing In Vivo with Ribozyme-Based Reporters","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Imaging Target mRNA and siRNA-Mediated Gene Silencing In Vivo with Ribozyme-Based Reporters"}]},"item_type_id":"8","owner":"1","path":["1"],"pubdate":{"attribute_name":"公開日","attribute_value":"2018-10-31"},"publish_date":"2018-10-31","publish_status":"0","recid":"49226","relation_version_is_last":true,"title":["Imaging Target mRNA and siRNA-Mediated Gene Silencing In Vivo with Ribozyme-Based Reporters"],"weko_creator_id":"1","weko_shared_id":-1},"updated":"2023-05-15T23:20:35.659996+00:00"}