@article{oai:repo.qst.go.jp:00049226,
 author = {So, Min-Kyung and Gowrishankar, Gayatri and Hasegawa, Sumitaka and et.al and 長谷川 純崇},
 issue = {16},
 journal = {Chembiochem},
 month = {Oct},
 note = {Noninvasive imaging of specific mRNAs in living subjects promises numerous biological and medical applications. Common strategies use fluorescently or radioactively labelled antisense probes to detect target mRNAs through a hybridization mechanism, but have met with limited success in living animals. Here
we present a novel molecular imaging approach based on the group I intron of Tetrahymena thermophila for imaging mRNA molecules in vivo. Engineered trans-splicing ribozyme reporters contain three domains, each of which is designed for targeting, splicing, and reporting. They can transduce the target mRNA into a reporter mRNA, leading to the production of reporter enzymes that can be noninvasively imaged in vivo. We have demonstrated this ribozyme-mediated RNA imaging method for imaging a mutant p53 mRNA both in single cells and noninvasively in living mice. After optimization, the ribozyme reporter increases contrast for the transiently expressed target by 180-fold, and by ten-fold for the stably expressed target. siRNA-mediated specific gene silencingof p53 expression has been successfully imaged in real time in vivo. This newribozyme-ba sed RNA reporter system should open up newavenues for in vivo RNA imaging and direct imaging of siRNA inhibition.},
 pages = {2682--2691},
 title = {Imaging Target mRNA and siRNA-Mediated Gene Silencing In Vivo with Ribozyme-Based Reporters},
 volume = {9},
 year = {2008}
}