{"created":"2023-05-15T14:38:08.218239+00:00","id":49206,"links":{},"metadata":{"_buckets":{"deposit":"5fbdbeee-131a-4ca5-b267-0b0472b40292"},"_deposit":{"created_by":1,"id":"49206","owners":[1],"pid":{"revision_id":0,"type":"depid","value":"49206"},"status":"published"},"_oai":{"id":"oai:repo.qst.go.jp:00049206","sets":["1"]},"author_link":["496453","496455","496451","496454","496450","496452","496456","496449"],"item_8_biblio_info_7":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2018-10","bibliographicIssueDateType":"Issued"},"bibliographicPageEnd":"1091","bibliographicPageStart":"1084","bibliographicVolumeNumber":"1866","bibliographic_titles":[{"bibliographic_title":"BBA Proteins and Proteomics"}]}]},"item_8_description_5":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"L-Arabinose isomerase isolated from Geobacillus stearothermophilus (GSAI) was modified to improve its substrate specificity for D-galactose for the production of D-tagatose, a potential reduced-energy sweetener. Among the selected residues, mutation at residue 18 produced a mutant strain, H18T, which exhibited increased activity for D-galactose compared with the wild-type (WT) enzyme. Analysis of the substrate specificity of H18T showed a 45.4% improvement for D-galactose. Replacing histidine with threonine at residue 18 resulted in approximately 2.7-fold and 1.8-fold higher substrate binding and catalytic efficiency, respectively, for D-galactose. Further enhancement of the specific activity and catalytic efficiency of H18T for D-galactose by up to 2.7-fold and 4.3- fold, respectively, was achieved by adding borate during L-arabinose isomerase catalysis. Moreover, H18T showed thermostability and no destabilization was detected, which is promising for the industrial production of D-tagatose","subitem_description_type":"Abstract"}]},"item_8_publisher_8":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"Elsevier Inc."}]},"item_8_relation_14":{"attribute_name":"DOI","attribute_value_mlt":[{"subitem_relation_type_id":{"subitem_relation_type_id_text":"10.1016/j.bbapap.2018.09.002","subitem_relation_type_select":"DOI"}}]},"item_8_source_id_9":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"1570-9639","subitem_source_identifier_type":"ISSN"}]},"item_access_right":{"attribute_name":"アクセス権","attribute_value_mlt":[{"subitem_access_right":"metadata only access","subitem_access_right_uri":"http://purl.org/coar/access_right/c_14cb"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Amreta, Laksmi Fina"}],"nameIdentifiers":[{"nameIdentifier":"496449","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"新井, 栄揮"}],"nameIdentifiers":[{"nameIdentifier":"496450","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Tsurumaru, Hirohito"}],"nameIdentifiers":[{"nameIdentifier":"496451","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Nakamura, Yoshitaka"}],"nameIdentifiers":[{"nameIdentifier":"496452","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Saksono, Budi"}],"nameIdentifiers":[{"nameIdentifier":"496453","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Tokunaga, Masao"}],"nameIdentifiers":[{"nameIdentifier":"496454","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Ishibashi, Matsujiro"}],"nameIdentifiers":[{"nameIdentifier":"496455","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"新井 栄揮","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"496456","nameIdentifierScheme":"WEKO"}]}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"Improved substrate specificity for D-galactose of L-arabinose isomerase for industrial application","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Improved substrate specificity for D-galactose of L-arabinose isomerase for industrial application"}]},"item_type_id":"8","owner":"1","path":["1"],"pubdate":{"attribute_name":"公開日","attribute_value":"2018-10-12"},"publish_date":"2018-10-12","publish_status":"0","recid":"49206","relation_version_is_last":true,"title":["Improved substrate specificity for D-galactose of L-arabinose isomerase for industrial application"],"weko_creator_id":"1","weko_shared_id":-1},"updated":"2023-05-15T23:20:43.653630+00:00"}