{"created":"2023-05-15T14:38:02.165635+00:00","id":49068,"links":{},"metadata":{"_buckets":{"deposit":"d056eabc-99ad-4232-9f8c-6cc14dfb41a7"},"_deposit":{"created_by":1,"id":"49068","owners":[1],"pid":{"revision_id":0,"type":"depid","value":"49068"},"status":"published"},"_oai":{"id":"oai:repo.qst.go.jp:00049068","sets":["1"]},"author_link":["795824","795822","795823","795827","795819","795821","795825","795826","795820"],"item_8_biblio_info_7":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2018-05","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"11","bibliographicPageEnd":"2562","bibliographicPageStart":"2554","bibliographicVolumeNumber":"9","bibliographic_titles":[{"bibliographic_title":"ACS chemical neuroscience"}]}]},"item_8_description_5":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"The dynamics of l-lactate transport across the blood-brain barrier (BBB) and its cerebral metabolism are still subject to debate. We studied lactate uptake and intracellular metabolism in the mouse brain using hyperpolarized C magnetic resonance spectroscopy (MRS). Following the intravenous injection of hyperpolarized [1-C]lactate, we observed that the distribution of the C label between lactate and pyruvate, which has been shown to be representative of their pool size ratio, is different in NMRI and C57BL/6 mice, the latter exhibiting a higher level of cerebral lactate dehydrogenase A ( Ldha) expression. On the basis of this observation, and an additional set of experiments showing that the cerebral conversion of [1-C]lactate to [1-C]pyruvate increases after exposing the brain to ultrasound irradiation that reversibly opens the BBB, we concluded that lactate transport is rate-limited by the BBB, with a 30% increase in lactate uptake after its disruption. It was also deduced from these results that hyperpolarized C MRS can be used to detect a variation in cerebral lactate uptake of <40 nmol in a healthy brain during an in vivo experiment lasting only 75 s, opening new opportunities to study the role of lactate in brain metabolism.","subitem_description_type":"Abstract"}]},"item_8_publisher_8":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"American Chemical Society."}]},"item_8_relation_13":{"attribute_name":"PubMed番号","attribute_value_mlt":[{"subitem_relation_type_id":{"subitem_relation_type_id_text":"29771492","subitem_relation_type_select":"PMID"}}]},"item_8_relation_14":{"attribute_name":"DOI","attribute_value_mlt":[{"subitem_relation_type_id":{"subitem_relation_type_id_text":"10.1021/acschemneuro.8b00066","subitem_relation_type_select":"DOI"}}]},"item_8_relation_17":{"attribute_name":"関連サイト","attribute_value_mlt":[{"subitem_relation_type_id":{"subitem_relation_type_id_text":"https://pubs.acs.org/doi/10.1021/acschemneuro.8b00066","subitem_relation_type_select":"URI"}}]},"item_8_source_id_9":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"1948-7193","subitem_source_identifier_type":"ISSN"}]},"item_access_right":{"attribute_name":"アクセス権","attribute_value_mlt":[{"subitem_access_right":"metadata only access","subitem_access_right_uri":"http://purl.org/coar/access_right/c_14cb"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Takado, Yuhei"}],"nameIdentifiers":[{"nameIdentifier":"795819","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Cheng, Tian"}],"nameIdentifiers":[{"nameIdentifier":"795820","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"A M Bastiaansen, Jessica"}],"nameIdentifiers":[{"nameIdentifier":"795821","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"A I Yoshihara, Hikari"}],"nameIdentifiers":[{"nameIdentifier":"795822","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Lanz, Bernard"}],"nameIdentifiers":[{"nameIdentifier":"795823","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Mishkovsky, Mor"}],"nameIdentifiers":[{"nameIdentifier":"795824","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Lengacher, Sylvain"}],"nameIdentifiers":[{"nameIdentifier":"795825","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Comment, Arnaud"}],"nameIdentifiers":[{"nameIdentifier":"795826","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Takado, Yuhei","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"795827","nameIdentifierScheme":"WEKO"}]}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"Hyperpolarized 13C magnetic resonance spectroscopy reveals the rate-limiting role of the blood-brain barrier in the cerebral uptake and metabolism of L-lactate in vivo.","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Hyperpolarized 13C magnetic resonance spectroscopy reveals the rate-limiting role of the blood-brain barrier in the cerebral uptake and metabolism of L-lactate in vivo."}]},"item_type_id":"8","owner":"1","path":["1"],"pubdate":{"attribute_name":"公開日","attribute_value":"2018-05-29"},"publish_date":"2018-05-29","publish_status":"0","recid":"49068","relation_version_is_last":true,"title":["Hyperpolarized 13C magnetic resonance spectroscopy reveals the rate-limiting role of the blood-brain barrier in the cerebral uptake and metabolism of L-lactate in vivo."],"weko_creator_id":"1","weko_shared_id":-1},"updated":"2023-05-15T23:55:17.353006+00:00"}