@article{oai:repo.qst.go.jp:00049068,
 author = {Takado, Yuhei and Cheng, Tian and A M Bastiaansen, Jessica and A I Yoshihara, Hikari and Lanz, Bernard and Mishkovsky, Mor and Lengacher, Sylvain and Comment, Arnaud and Takado, Yuhei},
 issue = {11},
 journal = {ACS chemical neuroscience},
 month = {May},
 note = {The dynamics of l-lactate transport across the blood-brain barrier (BBB) and its cerebral metabolism are still subject to debate. We studied lactate uptake and intracellular metabolism in the mouse brain using hyperpolarized C magnetic resonance spectroscopy (MRS). Following the intravenous injection of hyperpolarized [1-C]lactate, we observed that the distribution of the C label between lactate and pyruvate, which has been shown to be representative of their pool size ratio, is different in NMRI and C57BL/6 mice, the latter exhibiting a higher level of cerebral lactate dehydrogenase A ( Ldha) expression. On the basis of this observation, and an additional set of experiments showing that the cerebral conversion of [1-C]lactate to [1-C]pyruvate increases after exposing the brain to ultrasound irradiation that reversibly opens the BBB, we concluded that lactate transport is rate-limited by the BBB, with a 30% increase in lactate uptake after its disruption. It was also deduced from these results that hyperpolarized C MRS can be used to detect a variation in cerebral lactate uptake of <40 nmol in a healthy brain during an in vivo experiment lasting only 75 s, opening new opportunities to study the role of lactate in brain metabolism.},
 pages = {2554--2562},
 title = {Hyperpolarized 13C magnetic resonance spectroscopy reveals the rate-limiting role of the blood-brain barrier in the cerebral uptake and metabolism of L-lactate in vivo.},
 volume = {9},
 year = {2018}
}