{"created":"2023-05-15T14:38:01.629945+00:00","id":49056,"links":{},"metadata":{"_buckets":{"deposit":"7d741cdb-1fb3-4aa3-84a7-697fc26dbdf4"},"_deposit":{"created_by":1,"id":"49056","owners":[1],"pid":{"revision_id":0,"type":"depid","value":"49056"},"status":"published"},"_oai":{"id":"oai:repo.qst.go.jp:00049056","sets":["1"]},"author_link":["865056","865058","865054","865051","865060","865055","865057","865059","865053","865061","865052"],"item_8_biblio_info_7":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2018-05","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"5","bibliographicPageStart":"1497","bibliographicVolumeNumber":"19","bibliographic_titles":[{"bibliographic_title":"International Journal of Molecular Sciences"}]}]},"item_8_description_5":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"Neurofibrillary tangles composed of hyperphosphorylated tau protein are primarily\nneuropathological features of a number of neurodegenerative diseases collectively termed tauopathy.\nTo understand the mechanisms underlying the cause of tauopathy, precise cellular and animal models\nare required. Recent data suggest that the transient introduction of exogenous tau can accelerate the\ndevelopment of tauopathy in the brains of non-transgenic and transgenic mice expressing wild-type\nhuman tau. However, the transmission mechanism leading to tauopathy is not fully understood.\nIn this study, we developed cultured-cell models of tauopathy representing a human tauopathy.\nNeuro2a (N2a) cells containing propagative tau filaments were generated by introducing purified tau\nfibrils. These cell lines expressed full-length (2N4R) human tau and the green fluorescent protein\n(GFP)-fused repeat domain of tau with P301L mutation. Immunocytochemistry and super-resolution\nmicroscopic imaging revealed that tau inclusions exhibited filamentous morphology and were\ncomposed of both full-length and repeat domain fragment tau. Live-cell imaging analysis revealed\nthat filamentous tau inclusions are transmitted to daughter cells, resulting in yeast-prion-like\npropagation. By a standard method of tau preparation, both full-length tau and repeat domain\nfragments were recovered in sarkosyl insoluble fraction. Hyperphosphorylation of full-length tau\nwas confirmed by the immunoreactivity of phospho-Tau antibodies and mobility shifts by sodium\ndodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE). These properties were similar to\nthe biochemical features of P301L mutated human tau in a mouse model of tauopathy. In addition,\nfilamentous tau aggregates in cells barely co-localized with ubiquitins, suggesting that most tau\naggregates were excluded from protein degradation systems, and thus propagated to daughter cells.\nThe present cellular model of tauopathy will provide an advantage for dissecting the mechanisms of\ntau aggregation and degradation and be a powerful tool for drug screening to prevent tauopathy","subitem_description_type":"Abstract"}]},"item_8_publisher_8":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"MDPI"}]},"item_8_relation_14":{"attribute_name":"DOI","attribute_value_mlt":[{"subitem_relation_type_id":{"subitem_relation_type_id_text":"10.3390/ijms19051497 ","subitem_relation_type_select":"DOI"}}]},"item_8_relation_17":{"attribute_name":"関連サイト","attribute_value_mlt":[{"subitem_relation_type_id":{"subitem_relation_type_id_text":"https://www.mdpi.com/1422-0067/19/5/1497","subitem_relation_type_select":"URI"}}]},"item_8_source_id_9":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"1422-0067","subitem_source_identifier_type":"ISSN"}]},"item_access_right":{"attribute_name":"アクセス権","attribute_value_mlt":[{"subitem_access_right":"metadata only access","subitem_access_right_uri":"http://purl.org/coar/access_right/c_14cb"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Gen Matsumoto"}],"nameIdentifiers":[{"nameIdentifier":"865051","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Matsumoto, Kazuki"}],"nameIdentifiers":[{"nameIdentifier":"865052","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Kimura, Taeko"}],"nameIdentifiers":[{"nameIdentifier":"865053","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Suhara, Tetsuya"}],"nameIdentifiers":[{"nameIdentifier":"865054","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Higuchi, Makoto"}],"nameIdentifiers":[{"nameIdentifier":"865055","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Sahara, Naruhiko"}],"nameIdentifiers":[{"nameIdentifier":"865056","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Mori, Nozomu"}],"nameIdentifiers":[{"nameIdentifier":"865057","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Kimura, Taeko","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"865058","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Suhara, Tetsuya","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"865059","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Higuchi, Makoto","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"865060","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Sahara, Naruhiko","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"865061","nameIdentifierScheme":"WEKO"}]}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"Tau Fibril Formation in Cultured Cells Compatible with a Mouse Model of Tauopathy","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Tau Fibril Formation in Cultured Cells Compatible with a Mouse Model of Tauopathy"}]},"item_type_id":"8","owner":"1","path":["1"],"pubdate":{"attribute_name":"公開日","attribute_value":"2018-05-18"},"publish_date":"2018-05-18","publish_status":"0","recid":"49056","relation_version_is_last":true,"title":["Tau Fibril Formation in Cultured Cells Compatible with a Mouse Model of Tauopathy"],"weko_creator_id":"1","weko_shared_id":-1},"updated":"2023-05-15T21:59:20.431598+00:00"}