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[18F]FEDAC as a targeting agent for activated macrophages in DBA/1 mice with collagen-induced arthritis: Comparison with [18F]FDG
https://repo.qst.go.jp/records/49032
https://repo.qst.go.jp/records/490320616c9a3-7631-44e2-b21c-fcf61430a63e
| Item type | 学術雑誌論文 / Journal Article(1) | |||||
|---|---|---|---|---|---|---|
| 公開日 | 2018-05-17 | |||||
| タイトル | ||||||
| タイトル | [18F]FEDAC as a targeting agent for activated macrophages in DBA/1 mice with collagen-induced arthritis: Comparison with [18F]FDG | |||||
| 言語 | ||||||
| 言語 | eng | |||||
| 資源タイプ | ||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
| 資源タイプ | journal article | |||||
| アクセス権 | ||||||
| アクセス権 | metadata only access | |||||
| アクセス権URI | http://purl.org/coar/access_right/c_14cb | |||||
| 著者 |
Chung, Seock-Jin
× Chung, Seock-Jin× Hai-JeonYoon× Youn, Hyewon× Jeong, Kim Mi× Lee, Yun-Sang× Min, Jeong Jae× Chung, June-Key× Wook, Kang Keon× Xie, Lin× Zhang, Ming-Rong× Jeong, Cheon Gi× 謝 琳× 張 明栄 |
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| 抄録 | ||||||
| 内容記述タイプ | Abstract | |||||
| 内容記述 | Activated macrophages have been known to play pivotal roles in the pathogenesis of rheumatoid arthritis (RA). [18F]FEDAC is a radiolabeled ligand for the translocator protein TSPO, which is abundant in activated macrophages. We evaluated the feasibility of [18F]FEDAC in a murine RA model. Methods: RAW 264.7 mouse macrophages were activated by lipopolysaccharide. TSPO expression levels in activated and inactivated macrophages were measured by quantitative polymerase chain reaction (qPCR) and western blotting. The cellular uptake and specific binding of [18F]FEDAC were measured using a gamma counter. For the in vivo study, collagen-induced arthritis (CIA) was developed in DBA/1 mice and the clinical score for arthritis was measured regularly. [18F]FEDAC and [18F]FDG positron emission tomography (PET) images were acquired on days 23 and 37 after first immunization. Histological examinations were performed to evaluate macrophages and TSPO expression. Results: We found increased TSPO mRNA and protein expression in activated macrophages.Uptake of [18F]FEDAC in activated macrophages was higher than that in non-activated cells, and was successfully blocked by the competitor PK11195. In CIA mice, joint swelling was apparent on day 26 after the first immunization and the condition worsened by day 37. [18F]FEDAC uptake by arthritic joints increased early on (day 23), whereas [18F]FDG uptake did not. However, [18F]FDG uptake by arthritic joints markedly increased at later stages (day 37) to a higher level than [18F]FEDAC uptake. The [18F]FEDAC uptake was weakly correlated with summed clinical severity score (p=0.019, r=0.313), whereas the [18F]FDG uptake was strongly correlated with summed severity score (p<0.001, r=0.897). Immunohistochemical staining of the arthritic joint demonstrated an influx of macrophages compared to that in normal joints. Conclusions [18F]FEDAC enabled the visualization of active inflammation sites in arthritic joints in a CIA model by targeting TSPO expression in activated macrophages. The results suggest the potential usefulness of [18F]FEDAC imaging in early phase of RA. |
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| 書誌情報 |
Journal of Nuclear Medicine 巻 59, 号 5, p. 839-845, 発行日 2018-01 |
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| PubMed番号 | ||||||
| 識別子タイプ | PMID | |||||
| 関連識別子 | 29326355 | |||||
| DOI | ||||||
| 識別子タイプ | DOI | |||||
| 関連識別子 | 10.2967/jnumed.117.200667 | |||||
