@article{oai:repo.qst.go.jp:00048806,
 author = {Araki, Ryoko and Mizutani, Eiji and Hoki, Yuko and Sunayama, Misato and Wakayama, Sayaka and Nagatomo, Hiroaki and Kasama, Yasuji and Nakamura, Miki and Wakayama, Teruhiko and Abe, Masumi and 荒木 良子 and 藤森 ゆう子 and 砂山 美里 and 笠間 康次 and 中村 美樹 and 安倍 真澄},
 issue = {5},
 journal = {Stem Cells},
 month = {Mar},
 note = {Induced pluripotent stem cells hold great promise for regenerative medicine but point mutations have been identified in these cells and have raised serious concerns about their safe use.We generated nuclear transfer embryonic stem cells (ntESCs) from both mouse embryonic fibroblasts (MEFs) and tail-tip fibroblasts (TTFs) and by whole genome sequencing found fewer mutations compared with iPSCs generated by retroviral gene transduction. Furthermore, TTF-derived ntESCs showed only a very small number of point mutations, approximately 80% less than the number observed in iPSCs generated using retrovirus. Base substitution profile analysis confirmed this greatly reduced number of point mutations. The point mutations in iPSCs are therefore not a Yamanaka factor-specific phenomenon but are intrinsic to genome reprogramming. Moreover, the dramatic reduction in point mutations in ntESCs suggests that most are not essential for genome reprogramming. Our results suggest that it is feasible to reduce the point mutation frequency in iPSCs by optimizing various genome reprogramming conditions. We conducted whole genome sequencing of ntES cells derived from MEFs or TTFs. We thereby succeeded in establishing TTF-derived ntES cell lines with far fewer point
mutations. Base substitution profile analysis of these clones also indicated a reduced point mutation frequency, moving from a transversion-predominance to a transition-predominance.},
 pages = {1189--1196},
 title = {The Number of Point Mutations in Induced Pluripotent Stem Cells and Nuclear Transfer Embryonic Stem Cells Depends on the Method and Somatic Cell Type Used for Their Generation},
 volume = {35},
 year = {2017}
}