{"created":"2023-05-15T14:37:48.694847+00:00","id":48785,"links":{},"metadata":{"_buckets":{"deposit":"5e6ba9f5-13e0-407a-9806-ff54a35a5e3f"},"_deposit":{"created_by":1,"id":"48785","owners":[1],"pid":{"revision_id":0,"type":"depid","value":"48785"},"status":"published"},"_oai":{"id":"oai:repo.qst.go.jp:00048785","sets":["1"]},"author_link":["491217","491219","491224","491214","491209","491216","491218","491221","491213","491220","491222","491223","491212","491211","491215","491210","491225"],"item_8_biblio_info_7":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2018-01","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"723","bibliographicVolumeNumber":"11","bibliographic_titles":[{"bibliographic_title":"Frontiers Neuroscience"}]}]},"item_8_description_5":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"Green fluorescence imaging (e.g., flavoprotein autofluorescence imaging, FAI) can be\nused to measure neuronal activity and oxygen metabolism in living brains without\nexpressing fluorescence proteins. It is useful for understanding the mechanism of\nvarious brain functions and their abnormalities in age-related brain diseases. However,\nhemoglobin in cerebral blood vessels absorbs green fluorescence, hampering accurate\nassessments of brain function in animal models with cerebral blood vessel dysfunctions\nand subsequent cerebral blood flow (CBF) alterations. In the present study, we developed\na new method to correct FAI signals for hemoglobin-dependent green fluorescence\nreductions by simultaneous measurements of green fluorescence and intrinsic optical\nsignals. Intrinsic optical imaging enabled evaluations of light absorption and scatters\nby hemoglobin, which could then be applied to corrections of green fluorescence\nintensities. Using thismethod, enhanced flavoprotein autofluorescence by sensory stimuli\nwas successfully detected in the brains of awake mice, despite increases of CBF,\nand hemoglobin interference. Moreover, flavoprotein autofluorescence could be properly\nquantified in a resting state and during sensory stimulation by a CO2 inhalation challenge,\nwhich modified vascular responses without overtly affecting neuronal activities. The\nflavoprotein autofluorescence signal data obtained here were in good agreement with the\nprevious findings from a condition with drug-induced blockade of cerebral vasodilation,\njustifying the current assaying methodology. Application of this technology to studies on\nanimal models of brain diseases with possible changes of CBF, including age-related\nneurological disorders, would provide better understanding of the mechanisms of\nneurovascular coupling in pathological circumstances.","subitem_description_type":"Abstract"}]},"item_8_relation_13":{"attribute_name":"PubMed番号","attribute_value_mlt":[{"subitem_relation_type_id":{"subitem_relation_type_id_text":"29354026","subitem_relation_type_select":"PMID"}}]},"item_8_relation_14":{"attribute_name":"DOI","attribute_value_mlt":[{"subitem_relation_type_id":{"subitem_relation_type_id_text":"10.3389/fnins.2017.00723","subitem_relation_type_select":"DOI"}}]},"item_8_source_id_9":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"1662-453X","subitem_source_identifier_type":"ISSN"}]},"item_access_right":{"attribute_name":"アクセス権","attribute_value_mlt":[{"subitem_access_right":"metadata only access","subitem_access_right_uri":"http://purl.org/coar/access_right/c_14cb"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Manami, Takahashi"}],"nameIdentifiers":[{"nameIdentifier":"491209","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Urushihata, Takuya"}],"nameIdentifiers":[{"nameIdentifier":"491210","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Takuwa, Hiroyuki"}],"nameIdentifiers":[{"nameIdentifier":"491211","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Sakata, Kazumi"}],"nameIdentifiers":[{"nameIdentifier":"491212","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Takado, Yuhei"}],"nameIdentifiers":[{"nameIdentifier":"491213","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Shimizu, Eiji"}],"nameIdentifiers":[{"nameIdentifier":"491214","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Suhara, Tetsuya"}],"nameIdentifiers":[{"nameIdentifier":"491215","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Higuchi, Makoto"}],"nameIdentifiers":[{"nameIdentifier":"491216","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Ito, Hiroshi"}],"nameIdentifiers":[{"nameIdentifier":"491217","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"高橋 真奈美","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"491218","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"漆畑 拓弥","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"491219","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"田桑 弘之","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"491220","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"高堂 裕平","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"491221","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"清水 栄司","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"491222","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"須原 哲也","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"491223","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"樋口 真人","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"491224","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"伊藤 浩","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"491225","nameIdentifierScheme":"WEKO"}]}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"Imaging of neuronal activity in awake mice by measurements of flavoprotein autofluorescence corrected for cerebral blood flow","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Imaging of neuronal activity in awake mice by measurements of flavoprotein autofluorescence corrected for cerebral blood flow"}]},"item_type_id":"8","owner":"1","path":["1"],"pubdate":{"attribute_name":"公開日","attribute_value":"2018-04-23"},"publish_date":"2018-04-23","publish_status":"0","recid":"48785","relation_version_is_last":true,"title":["Imaging of neuronal activity in awake mice by measurements of flavoprotein autofluorescence corrected for cerebral blood flow"],"weko_creator_id":"1","weko_shared_id":-1},"updated":"2023-05-15T23:24:19.971987+00:00"}