{"created":"2023-05-15T14:37:42.874963+00:00","id":48652,"links":{},"metadata":{"_buckets":{"deposit":"a03d97b6-3dea-4b63-9f13-f0f65582c591"},"_deposit":{"created_by":1,"id":"48652","owners":[1],"pid":{"revision_id":0,"type":"depid","value":"48652"},"status":"published"},"_oai":{"id":"oai:repo.qst.go.jp:00048652","sets":["1"]},"author_link":["489358","489361","489357","489362","489360","489359","489356"],"item_8_biblio_info_7":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2018-01","bibliographicIssueDateType":"Issued"},"bibliographicPageEnd":"1-8","bibliographicPageStart":"1-1","bibliographicVolumeNumber":"40","bibliographic_titles":[{"bibliographic_title":"Genes and Environment"}]}]},"item_8_description_5":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"Environmental factors stimulate alteration of DNA methylation level. Investigation of the genome-wide DNA methylation status is important for environmental health studies. We here designed a genomic DNA amplification and labeling protocol using a methylation-sensitive restriction enzyme HinP1 I. This method can specifically amplify genomic DNA fragments possessing methyl-CpG at the end. The fragments are a relatively short size and dominantly located on CpG-islands. By using the samples prepared by this method, a dioxin-induced change in the methylation level of the mouse Cyp1a1 promoter was successfully evaluated using oligonucleotide probes covalently bound onto a glass plate. The method developed in this paper would be useful for other genome-wide analysis platforms for the large scale epigenome-wide association studies (EWAS) including human epidemiological samples.","subitem_description_type":"Abstract"}]},"item_8_relation_14":{"attribute_name":"DOI","attribute_value_mlt":[{"subitem_relation_type_id":{"subitem_relation_type_id_text":"10.1186/s41021-017-0089-5","subitem_relation_type_select":"DOI"}}]},"item_access_right":{"attribute_name":"アクセス権","attribute_value_mlt":[{"subitem_access_right":"metadata only access","subitem_access_right_uri":"http://purl.org/coar/access_right/c_14cb"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"H, Kurita"}],"nameIdentifiers":[{"nameIdentifier":"489356","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Aiba, Toshiki"}],"nameIdentifiers":[{"nameIdentifier":"489357","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Saito, Toshiyuki"}],"nameIdentifiers":[{"nameIdentifier":"489358","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Ohsako, Seiichiroh"}],"nameIdentifiers":[{"nameIdentifier":"489359","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"相場 俊樹","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"489360","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"齋藤 俊行","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"489361","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"大迫 誠一郎","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"489362","nameIdentifierScheme":"WEKO"}]}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"Detection of dioxin-induced demethylation of mouse Cyp1a1 gene promoter by a new labeling method for short DNA fragments possessing 5'-methylcytosine at the end.","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Detection of dioxin-induced demethylation of mouse Cyp1a1 gene promoter by a new labeling method for short DNA fragments possessing 5'-methylcytosine at the end."}]},"item_type_id":"8","owner":"1","path":["1"],"pubdate":{"attribute_name":"公開日","attribute_value":"2018-03-27"},"publish_date":"2018-03-27","publish_status":"0","recid":"48652","relation_version_is_last":true,"title":["Detection of dioxin-induced demethylation of mouse Cyp1a1 gene promoter by a new labeling method for short DNA fragments possessing 5'-methylcytosine at the end."],"weko_creator_id":"1","weko_shared_id":-1},"updated":"2023-05-15T23:25:47.755565+00:00"}