{"created":"2023-05-15T14:37:34.353889+00:00","id":48467,"links":{},"metadata":{"_buckets":{"deposit":"ed2c8724-a913-4f9d-bf5c-22fa7775d771"},"_deposit":{"created_by":1,"id":"48467","owners":[1],"pid":{"revision_id":0,"type":"depid","value":"48467"},"status":"published"},"_oai":{"id":"oai:repo.qst.go.jp:00048467","sets":["1"]},"author_link":["487268","487267","487266","487271","487269","487272","487265","487270"],"item_8_biblio_info_7":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2018-01","bibliographicIssueDateType":"Issued"},"bibliographicPageEnd":"1394-10","bibliographicPageStart":"1394-1","bibliographicVolumeNumber":"8","bibliographic_titles":[{"bibliographic_title":"Scientific Reports"}]}]},"item_8_description_5":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"Ionizing radiation including heavy-ion beams has been widely used in plant mutation breeding. Dry seeds, seedlings, and cultured tissues are often used for mutagenesis; however, little is known about the differences in induced mutations among them. Here, we examined the characteristics of mutations using randomly chosen Arabidopsis M2 plants derived from dry seeds and seedlings irradiated with carbon ions. The mutation frequency was 1.4–1.9 times higher in dry-seed irradiation than in seedling irradiation. This difference was mainly due to the three-times higher frequency of insertions and deletions (InDels) in dry-seed irradiation than in seedling irradiation. This difference increased the proportion of mutations predicted to affect gene function among all mutations identified by whole genome re-sequencing. Our results demonstrate that the physiological status of plant tissue greatly affects the characteristics of mutations induced by ionizing radiation, and that dry seeds are more suitable materials than seedlings for inducing loss-of-function mutations. The results also showed that single base deletions often occurred in homopolymeric sequences, while InDels larger than 2–3 bp often occurred in or near polynucleotide-repeat or microhomologous sequences. Interestingly, microhomology was less commonly found around large deletions (≥50 bp), suggesting that the rejoining process differs depending on the deletion size. ","subitem_description_type":"Abstract"}]},"item_8_relation_14":{"attribute_name":"DOI","attribute_value_mlt":[{"subitem_relation_type_id":{"subitem_relation_type_id_text":"10.1038/s41598-018-19278-1","subitem_relation_type_select":"DOI"}}]},"item_access_right":{"attribute_name":"アクセス権","attribute_value_mlt":[{"subitem_access_right":"metadata only access","subitem_access_right_uri":"http://purl.org/coar/access_right/c_14cb"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"長谷, 純宏"}],"nameIdentifiers":[{"nameIdentifier":"487265","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"佐藤, 勝也"}],"nameIdentifiers":[{"nameIdentifier":"487266","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"北村, 智"}],"nameIdentifiers":[{"nameIdentifier":"487267","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"大野, 豊"}],"nameIdentifiers":[{"nameIdentifier":"487268","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"長谷 純宏","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"487269","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"佐藤 勝也","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"487270","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"北村 智","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"487271","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"大野 豊","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"487272","nameIdentifierScheme":"WEKO"}]}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"Physiological status of plant tissue affects the frequency and types of mutations induced by carbon-ion irradiation in Arabidopsis","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Physiological status of plant tissue affects the frequency and types of mutations induced by carbon-ion irradiation in Arabidopsis"}]},"item_type_id":"8","owner":"1","path":["1"],"pubdate":{"attribute_name":"公開日","attribute_value":"2018-02-06"},"publish_date":"2018-02-06","publish_status":"0","recid":"48467","relation_version_is_last":true,"title":["Physiological status of plant tissue affects the frequency and types of mutations induced by carbon-ion irradiation in Arabidopsis"],"weko_creator_id":"1","weko_shared_id":-1},"updated":"2023-05-15T23:27:44.618484+00:00"}