{"created":"2023-05-15T14:37:31.363739+00:00","id":48400,"links":{},"metadata":{"_buckets":{"deposit":"faaadc95-4bdc-477e-95fc-cc86a018e570"},"_deposit":{"created_by":1,"id":"48400","owners":[1],"pid":{"revision_id":0,"type":"depid","value":"48400"},"status":"published"},"_oai":{"id":"oai:repo.qst.go.jp:00048400","sets":["1"]},"author_link":["486489","486485","486484","486483","486490","486488","486487","486481","486486","486482"],"item_8_biblio_info_7":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2018-01","bibliographicIssueDateType":"Issued"},"bibliographicPageEnd":"89","bibliographicPageStart":"84","bibliographicVolumeNumber":"38","bibliographic_titles":[{"bibliographic_title":"Phytomedicine"}]}]},"item_8_description_5":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"Background: Apocynin is a constituent of the medicinal herb Picrorhiza kurroa. It is an inhibitor of nicotinamide\nadenine dinucleotide phosphate oxidase. This compound shows potential anti-inflammatory and antioxidant\neffects and has been tested as a neuroprotectant in many animal models of brain disease. In such studies, understanding\nthe brain kinetics of apocynin would be important for interpreting its in vivo efficacy; however, little\nhas been reported on the kinetics of apocynin in the brain.\nPurpose: The purpose of this study is to investigate the kinetics and metabolism of apocynin in the brain of mice.\nStudy design: The kinetics and metabolism of apocynin were examined using [11C]apocynin and positronemission\ntomography (PET).\nMethods: In vivo PET scanning was performed in mice for 20 min after intraperitoneal administration of an\napocynin solution containing [11C]apocynin. Metabolites in the brain were analyzed using high-performance\nliquid chromatography. The doses of apocynin used ranged from <1.5 μg/kg (tracer dose) to 100 mg/kg.\nResults: Brain radioactivity during the period of 0 to 20 min after administration was negligible at the tracer dose\nand extremely low at the dose of 10 mg/kg. Moderate radioactivity was observed in the brain a few minutes after\nadministration at the doses of 25 and 50 mg/kg and rapidly decreased thereafter. At a dose of 100 mg/kg, [11C]\napocynin resulted in a high uptake of radioactivity followed by a gradual washout. In contrast to the brain, a\nclear dose-dependent increase in radioactivity was not observed in the blood. The fraction of the unchanged\nform in the brain decreased with time, and the degree of the reduction depended on apocynin doses: apocynin\nwas rapidly metabolized in the brain at lower doses, whereas it was slowly decomposed at higher doses. On the\nbasis of these data, the maximum apocynin concentrations in the brain were calculated to be 10 μM (10 mg/kg),\n49 μM (25 mg/kg), 150 μM (50 mg/kg), and 380 μM (100 mg/kg). A metabolite observed in the brain was found\nto be apocynin glucuronide but not diapocynin, an active metabolite.\nConclusion: These results would be useful for an evaluation of the potential efficacy of apocynin as a neuroprotective\nagent.","subitem_description_type":"Abstract"}]},"item_8_publisher_8":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"ELSEVIER"}]},"item_8_relation_14":{"attribute_name":"DOI","attribute_value_mlt":[{"subitem_relation_type_id":{"subitem_relation_type_id_text":"10.1016/j.phymed.2017.05.006","subitem_relation_type_select":"DOI"}}]},"item_8_source_id_9":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"0944-7113","subitem_source_identifier_type":"ISSN"}]},"item_access_right":{"attribute_name":"アクセス権","attribute_value_mlt":[{"subitem_access_right":"metadata only access","subitem_access_right_uri":"http://purl.org/coar/access_right/c_14cb"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Okamura, Toshimitsu"}],"nameIdentifiers":[{"nameIdentifier":"486481","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Okada, Maki"}],"nameIdentifiers":[{"nameIdentifier":"486482","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Kikuchi, Tatsuya"}],"nameIdentifiers":[{"nameIdentifier":"486483","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Wakizaka, Hidekatsu"}],"nameIdentifiers":[{"nameIdentifier":"486484","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Zhang, Ming-Rong"}],"nameIdentifiers":[{"nameIdentifier":"486485","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"岡村 敏充","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"486486","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"岡田 真希","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"486487","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"菊池 達矢","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"486488","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"脇坂 秀克","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"486489","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"張 明栄","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"486490","nameIdentifierScheme":"WEKO"}]}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"Kinetics and metabolism of apocynin in the mouse brain assessed with positron-emission tomography","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Kinetics and metabolism of apocynin in the mouse brain assessed with positron-emission tomography"}]},"item_type_id":"8","owner":"1","path":["1"],"pubdate":{"attribute_name":"公開日","attribute_value":"2017-12-05"},"publish_date":"2017-12-05","publish_status":"0","recid":"48400","relation_version_is_last":true,"title":["Kinetics and metabolism of apocynin in the mouse brain assessed with positron-emission tomography"],"weko_creator_id":"1","weko_shared_id":-1},"updated":"2023-05-15T23:28:32.620039+00:00"}