@article{oai:repo.qst.go.jp:00048367, author = {山原, 有未 (Yumi Yamahara) and 長澤, 尚胤 and 田口, 光正 and 大島, 明博 (Akihiro Oshima) and 鷲尾, 方一 (Masakazu Washio) and 長澤 尚胤 and 田口 光正}, journal = {Radiation Physics and Chemistry}, month = {Oct}, note = {Thermo-responsive templates for the cell cultivation based on Poly(tetrafluoroethylene-co-ethylene) (ETFE) were fabricated by pre-irradiation grafting of N-isoproplyacrylamide (NIPAAm) monomer by electron beam (EB) irradiation under nitrogen gas atmosphere at room temperature, and their characteristic properties were studied. The detachment of cultured HeLa cells from fabricated thermo-responsive templates were attempted. Furthermore, the reaction mechanism is proposed using ESR spectroscopy and FT-IR spectroscopy. It is confirmed that the cultured HeLa cells were detached from fabricated thermo-responsive templates at 20 °C. Water contact angle analysis indicated that obtained templates had thermo-response around 30 °C. It is suggested that the grafted polymer chains would mainly react with peroxy radicals (–CF2–CF(OO・)–) on tetrafluoroethylene unit in ETFE}, pages = {88--93}, title = {Fabrication of thermo-responsive PNIPAAm-g-ETFE for cell culture dishes by pre-irradiation grafting}, volume = {142}, year = {2017} }