{"created":"2023-05-15T14:37:20.509385+00:00","id":48152,"links":{},"metadata":{"_buckets":{"deposit":"564b0f3a-5a35-433b-a49a-a068d69b8068"},"_deposit":{"created_by":1,"id":"48152","owners":[1],"pid":{"revision_id":0,"type":"depid","value":"48152"},"status":"published"},"_oai":{"id":"oai:repo.qst.go.jp:00048152","sets":["1"]},"author_link":["483546","483550","483556","483547","483552","483544","483548","483551","483553","483554","483549","483555","483545"],"item_8_biblio_info_7":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2016-12","bibliographicIssueDateType":"Issued"},"bibliographicPageEnd":"158","bibliographicPageStart":"150","bibliographicVolumeNumber":"87","bibliographic_titles":[{"bibliographic_title":"Vascular Pharmacology"}]}]},"item_8_description_5":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"Plasminogen activator inhibitor-1 (PAI-1) is induced by radiation resulting in endothelial cell impairment, potentially leading to multiple organ failure. Vitronectin (VN) is a 75-kDa glycoprotein (VN75) cleaved into two forms (VN75 or VN65/10) by furin, which is regulated by intracellular PAI-1. VN protects against radiation-induced endothelial cell death, but the mechanisms involved in VN processing and its interactions with intra- and extracellular PAI-1 remain unclear. We examined these processes in cells in vitro using recombinant proteins or overexpression of VN and PAI-1 genes, including furin-susceptible (T381) and furin-resistant VN (A381). VN processing was analyzed using a mutant PAI-1 with relatively weaker binding to VN. VN function was evaluated by survival of radiation-damaged endothelial cells. Wild-type, but not mutant PAI-1 inhibited furin-dependent VN processing. Gene transfer revealed that furin-susceptible VN was processed more than the furin-resistant form, but processing of both was inhibited by PAI-1 overexpression. Intracellular PAI-1 formed a complex with VN75 (T381) in cells and media, and the VN75 form was secreted preferentially. Only VN75 protected against radiation-induced endothelial cell death, in which its effect was abolished by wild-type but not mutant PAI-1. These findings indicate that intracellular PAI-1 inhibits VN processing and protects against radiation-induced endothelial cell death.","subitem_description_type":"Abstract"}]},"item_8_publisher_8":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"Elsevier"}]},"item_8_relation_14":{"attribute_name":"DOI","attribute_value_mlt":[{"subitem_relation_type_id":{"subitem_relation_type_id_text":"10.1016/j.vph.2016.09.006","subitem_relation_type_select":"DOI"}}]},"item_access_right":{"attribute_name":"アクセス権","attribute_value_mlt":[{"subitem_access_right":"metadata only access","subitem_access_right_uri":"http://purl.org/coar/access_right/c_14cb"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Hazawa, Masaharu"}],"nameIdentifiers":[{"nameIdentifier":"483544","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Yasuda, Takeshi"}],"nameIdentifiers":[{"nameIdentifier":"483545","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Saotome-Nakamura, Ai"}],"nameIdentifiers":[{"nameIdentifier":"483546","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Tomiyama, Kenichi"}],"nameIdentifiers":[{"nameIdentifier":"483547","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Chizuka, Obara"}],"nameIdentifiers":[{"nameIdentifier":"483548","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Goto, Takaya"}],"nameIdentifiers":[{"nameIdentifier":"483549","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Katsushi, Tajima"}],"nameIdentifiers":[{"nameIdentifier":"483550","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"羽澤 勝治","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"483551","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"安田 武嗣","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"483552","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"富山 健一","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"483553","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"小原 千寿香","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"483554","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"後藤 孝也","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"483555","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"田嶋 克史","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"483556","nameIdentifierScheme":"WEKO"}]}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"Intra- and extracellular plasminogen activator inhibitor-1 regulate effect of vitronectin against radiation-induced endothelial cell death","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Intra- and extracellular plasminogen activator inhibitor-1 regulate effect of vitronectin against radiation-induced endothelial cell death"}]},"item_type_id":"8","owner":"1","path":["1"],"pubdate":{"attribute_name":"公開日","attribute_value":"2017-07-24"},"publish_date":"2017-07-24","publish_status":"0","recid":"48152","relation_version_is_last":true,"title":["Intra- and extracellular plasminogen activator inhibitor-1 regulate effect of vitronectin against radiation-induced endothelial cell death"],"weko_creator_id":"1","weko_shared_id":-1},"updated":"2023-05-15T23:31:20.978995+00:00"}