{"created":"2023-05-15T14:37:19.467472+00:00","id":48128,"links":{},"metadata":{"_buckets":{"deposit":"70726d29-1020-4745-8b38-b703edf0cea9"},"_deposit":{"created_by":1,"id":"48128","owners":[1],"pid":{"revision_id":0,"type":"depid","value":"48128"},"status":"published"},"_oai":{"id":"oai:repo.qst.go.jp:00048128","sets":["1"]},"author_link":["483277","483270","483274","483268","483265","483276","483271","483269","483267","483266","483272","483273","483275"],"item_8_biblio_info_7":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2016-04","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"1","bibliographicPageEnd":"127","bibliographicPageStart":"113","bibliographicVolumeNumber":"366","bibliographic_titles":[{"bibliographic_title":"Cell and tissue research"}]}]},"item_8_description_5":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"Three-dimensional (3-D) aggregate culturing is useful for investigating the functional properties of mesenchymal stem/stromal cells (MSCs). For 3-D MSC analysis, however, pre-expansion of MSCs with two-dimensional (2-D) monolayer culturing must first be performed, which might abolish their endogenous properties. To avoid the need for 2-D expansion, we used prospectively isolated mouse bone marrow (BM)-MSCs and examined the differences in the biological properties of 2-D and 3-D MSC cultures. The BM-MSCs self-assembled into aggregates on nanoculture plates (NCP) that have nanoimprinted patterns with a low-cellular binding texture. The 3-D MSCs proliferated at the same rate as 2-D-cultured cells by only diffusion culture and secreted higher levels of pro-angiogenic factors such as vascular endothelial growth factor and hepatocyte growth factor (HGF). Conditioned medium from 3-D MSC cultures promoted more capillary formation than that of 2-D MSCs in an in vitro tube formation assay. Matrigel-implanted 3-D MSC aggregates tended to induce angiogenesis in host mice. The 3-D culturing on NCP induced alpha-fetoprotein (AFP) expression in MSCs without the application of AFP- or endodermal-inducible factors, possibly via an HGF-autocrine mechanism, and maintained their differentiation ability for adipocytes, osteocytes, and chondrocytes. Prospectively isolated mouse BM-MSCs expressed low/negative stemness-related genes including Oct3/4, Nanog, and Sox2, which were not enhanced by NCP-based 3-D culturing, suggesting that some of these cells differentiate into meso-endodermal layer cells. Culturing of prospectively isolated MSCs on NCP in 3-D allows the analysis of the biological properties of more closely endogenous BM-MSCs and might contribute to tissue engineering and repair.","subitem_description_type":"Abstract"}]},"item_8_publisher_8":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"Springer Berlin Heidelberg"}]},"item_8_relation_14":{"attribute_name":"DOI","attribute_value_mlt":[{"subitem_relation_type_id":{"subitem_relation_type_id_text":"10.1007/s00441-016-2405-y","subitem_relation_type_select":"DOI"}}]},"item_8_relation_17":{"attribute_name":"関連サイト","attribute_value_mlt":[{"subitem_relation_name":[{"subitem_relation_name_text":"http://link.springer.com/article/10.1007%2Fs00441-016-2405-y"}],"subitem_relation_type_id":{"subitem_relation_type_id_text":"http://link.springer.com/article/10.1007%2Fs00441-016-2405-y","subitem_relation_type_select":"URI"}}]},"item_access_right":{"attribute_name":"アクセス権","attribute_value_mlt":[{"subitem_access_right":"metadata only access","subitem_access_right_uri":"http://purl.org/coar/access_right/c_14cb"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Obara, Chizuka"}],"nameIdentifiers":[{"nameIdentifier":"483265","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Tomiyama, Kenichi"}],"nameIdentifiers":[{"nameIdentifier":"483266","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Takizawa, Kazuya"}],"nameIdentifiers":[{"nameIdentifier":"483267","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Rafiqul, Isram"}],"nameIdentifiers":[{"nameIdentifier":"483268","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Yasuda, Takeshi"}],"nameIdentifiers":[{"nameIdentifier":"483269","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Gotoh, Takaya"}],"nameIdentifiers":[{"nameIdentifier":"483270","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Tajima, Katsushi"}],"nameIdentifiers":[{"nameIdentifier":"483271","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"小原 千寿香","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"483272","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"富山 健一","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"483273","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"滝澤 和也","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"483274","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"安田 武嗣","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"483275","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"後藤 孝也","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"483276","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"田嶋 克史","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"483277","nameIdentifierScheme":"WEKO"}]}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"Characteristics of three-dimensional prospectively isolated mouse bone marrow mesenchymal stem/stromal cell aggregates on nanoculture plates","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Characteristics of three-dimensional prospectively isolated mouse bone marrow mesenchymal stem/stromal cell aggregates on nanoculture plates"}]},"item_type_id":"8","owner":"1","path":["1"],"pubdate":{"attribute_name":"公開日","attribute_value":"2017-07-20"},"publish_date":"2017-07-20","publish_status":"0","recid":"48128","relation_version_is_last":true,"title":["Characteristics of three-dimensional prospectively isolated mouse bone marrow mesenchymal stem/stromal cell aggregates on nanoculture plates"],"weko_creator_id":"1","weko_shared_id":-1},"updated":"2023-05-15T23:31:38.677112+00:00"}