{"created":"2023-05-15T14:37:14.005027+00:00","id":48002,"links":{},"metadata":{"_buckets":{"deposit":"0833ad47-dd6b-4813-b294-7a12ff007afe"},"_deposit":{"created_by":1,"id":"48002","owners":[1],"pid":{"revision_id":0,"type":"depid","value":"48002"},"status":"published"},"_oai":{"id":"oai:repo.qst.go.jp:00048002","sets":["1"]},"author_link":["481856","481852","481855","481853","481854"],"item_8_biblio_info_7":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2016-10","bibliographicIssueDateType":"Issued"},"bibliographicPageEnd":"135","bibliographicPageStart":"129","bibliographicVolumeNumber":"510","bibliographic_titles":[{"bibliographic_title":"Analytical Biochemistry"}]}]},"item_8_description_5":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"The mutagenicity of clustered DNA damage can be analyzed with plasmids carrying a clustered DNA damage site, in which the strand bias of a replicating plasmid (i.e., the degree to which each of the two strands of the plasmid are used as the template for replication of the plasmid) can help to clarify how clustered DNA damage enhances the mutagenic potential of comprising lesions. Placement of a mismatch near a clustered DNA damage site can help to determine the strand bias, but present plasmid-based methods do not allow insertion of a mismatch at a given site in the plasmid. Here, we describe a polymerization-based method for constructing a plasmid containing clustered DNA lesions and a mismatch. The presence of a DNA lesion and a mismatch in the plasmid was verified by enzymatic treatment and by determining the relative abundance of the progeny plasmids derived from each of the two strands of the plasmid. ","subitem_description_type":"Abstract"}]},"item_8_publisher_8":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"Elsevier"}]},"item_8_relation_14":{"attribute_name":"DOI","attribute_value_mlt":[{"subitem_relation_type_id":{"subitem_relation_type_id_text":"10.1016/j.ab.2016.07.007","subitem_relation_type_select":"DOI"}}]},"item_access_right":{"attribute_name":"アクセス権","attribute_value_mlt":[{"subitem_access_right":"metadata only access","subitem_access_right_uri":"http://purl.org/coar/access_right/c_14cb"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Takahashi, Momoko"}],"nameIdentifiers":[{"nameIdentifier":"481852","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Akamatsu, Ken"}],"nameIdentifiers":[{"nameIdentifier":"481853","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Shikazono, Naoya"}],"nameIdentifiers":[{"nameIdentifier":"481854","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"赤松 憲","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"481855","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"鹿園 直哉","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"481856","nameIdentifierScheme":"WEKO"}]}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"A polymerization-based method to construct a plasmid containing clustered DNA damage and a mismatch","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"A polymerization-based method to construct a plasmid containing clustered DNA damage and a mismatch"}]},"item_type_id":"8","owner":"1","path":["1"],"pubdate":{"attribute_name":"公開日","attribute_value":"2017-06-05"},"publish_date":"2017-06-05","publish_status":"0","recid":"48002","relation_version_is_last":true,"title":["A polymerization-based method to construct a plasmid containing clustered DNA damage and a mismatch"],"weko_creator_id":"1","weko_shared_id":-1},"updated":"2023-05-15T23:33:08.168315+00:00"}