{"created":"2023-05-15T14:37:01.523280+00:00","id":47716,"links":{},"metadata":{"_buckets":{"deposit":"dad78967-a28e-42ad-9f39-7dfde3755170"},"_deposit":{"created_by":1,"id":"47716","owners":[1],"pid":{"revision_id":0,"type":"depid","value":"47716"},"status":"published"},"_oai":{"id":"oai:repo.qst.go.jp:00047716","sets":["1"]},"author_link":["478800","478796","478795","478799","478797","478798","478803","478801","478802"],"item_8_biblio_info_7":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2017-03","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"3","bibliographicPageEnd":"302","bibliographicPageStart":"295","bibliographicVolumeNumber":"93","bibliographic_titles":[{"bibliographic_title":"International journal of radiation biology"}]}]},"item_8_description_5":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"PURPOSE:\nTo clarify whether initial base excision repair processes at clustered DNA damage sites comprising multiple base lesions affect subsequent excision processes via the formation of additional strand breaks by glycosylase and apurinic/apyrimidinic (AP) endonuclease base excision enzymes.\nMATERIALS AND METHODS:\nPlasmid DNA (pUC18) as a model DNA molecule was exposed to high-linear-energy-transfer (LET) ionizing radiation (He2+ or C6+ ions) or low-LET ionizing radiation (X-rays) under various conditions to produce varied radical-scavenging effects. pUC18 was then treated sequentially or simultaneously with two bacterial base excision enzymes (glycosylases), namely, endonuclease III and formamidopyrimidine-DNA glycosylase, which convert pyrimidine (or abasic [AP] site) and purine (or AP site) lesions to single-strand breaks (SSB), respectively. Yields of additional SSB or double-strand breaks (DSB) as digestion products were examined after changing the order of enzymatic treatment.\nRESULTS:\nThere were few differences among the enzymatic treatments, indicating that treatment order did not affect the final yields of additional SSB or DSB formed by glycosylase activity. This suggests that of the total damage, the fraction of clustered damage sites with a persistent base lesion dependent on the order of glycosylase treatment was insignificant if present.\nCONCLUSION:\nBase lesion clusters induced by high- or low-LET radiation appear three or more base pairs apart, and are promptly converted to a DSB by glycosylase, regardless of the order of enzymatic treatment.","subitem_description_type":"Abstract"}]},"item_8_publisher_8":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"Taylor and Francis"}]},"item_8_relation_13":{"attribute_name":"PubMed番号","attribute_value_mlt":[{"subitem_relation_type_id":{"subitem_relation_type_id_text":"27707033","subitem_relation_type_select":"PMID"}}]},"item_8_relation_14":{"attribute_name":"DOI","attribute_value_mlt":[{"subitem_relation_type_id":{"subitem_relation_type_id_text":"10.1080/09553002.2017.1239849","subitem_relation_type_select":"DOI"}}]},"item_8_source_id_9":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"0955-3002","subitem_source_identifier_type":"ISSN"}]},"item_access_right":{"attribute_name":"アクセス権","attribute_value_mlt":[{"subitem_access_right":"metadata only access","subitem_access_right_uri":"http://purl.org/coar/access_right/c_14cb"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Shiraishi, Iyo"}],"nameIdentifiers":[{"nameIdentifier":"478795","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Shikazono, Naoya"}],"nameIdentifiers":[{"nameIdentifier":"478796","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Suzuki, Masao"}],"nameIdentifiers":[{"nameIdentifier":"478797","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Fujii, Kentaro"}],"nameIdentifiers":[{"nameIdentifier":"478798","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Yokoya, Akinari"}],"nameIdentifiers":[{"nameIdentifier":"478799","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"鹿園 直哉","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"478800","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"鈴木 雅雄","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"478801","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"藤井 健太郎","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"478802","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"横谷 明徳","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"478803","nameIdentifierScheme":"WEKO"}]}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"Efficiency of radiation-induced base lesion excision and the order of enzymatic treatment.","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Efficiency of radiation-induced base lesion excision and the order of enzymatic treatment."}]},"item_type_id":"8","owner":"1","path":["1"],"pubdate":{"attribute_name":"公開日","attribute_value":"2017-04-19"},"publish_date":"2017-04-19","publish_status":"0","recid":"47716","relation_version_is_last":true,"title":["Efficiency of radiation-induced base lesion excision and the order of enzymatic treatment."],"weko_creator_id":"1","weko_shared_id":-1},"updated":"2023-05-15T23:36:20.261350+00:00"}