@article{oai:repo.qst.go.jp:00047663,
 author = {Michikawa, Yuichi and Sugahara, Keisuke and Suga, Tomo and Ohtsuka, Yoshimi and Ishikawa, Kenichi and Ishikawa, Atsuko and Shiomi, Naoko and Shiomi, Tadahiro and Iwakawa, Mayumi and Imai, Takashi and 道川 祐市 and 菅原 圭亮 and 菅 智 and 荘司 好美 and 石川 顕一 and 石川 敦子 and 塩見 尚子 and 塩見 忠博 and 岩川 眞由美 and 今井 高志},
 issue = {2},
 journal = {Analytical Biochemistry},
 month = {Aug},
 note = {The isolation and multiple genotyping of long individual DNA fragments are needed to obtain haplotype information for diploid organisms. Limiting dilution of sample DNA followed by multiple displacement amplification is a useful technique but is restricted to short (<5 kb) DNA fragments. In the current study, a novel modification was applied to overcome these problems. A limited amount of cellular DNA was carefully released from intact cells into a mildly heated alkaline agarose solution and mixed thoroughly. The solution was then gently aliquoted and allowed to solidify while maintaining the integrity of the diluted DNA. Exogenously provided Phi29 DNA polymerase was used to perform consistent genomic amplification with random hexameric oligonucleotides within the agarose gels. Simple heat melting of the gel allowed recovery of the amplified materials in a solution of the polymerase chain reaction (PCR)-ready form. The haplotypes of seven SNPs spanning 240 kb of the DNA surrounding the human ATM gene region on chromosome 11 were determined for 10 individuals, demonstrating the feasibility of this new method.},
 pages = {151--158},
 title = {In-gel multiple displacement amplification of long DNA fragments diluted to the single molecule level},
 volume = {383},
 year = {2008}
}