@article{oai:repo.qst.go.jp:00047662,
 author = {Michikawa, Yuichi and Suga, Tomo and Ishikawa, Atsuko and Hayashi, Hideki and Oka, Akira and Inoko, Hidetoshi and Iwakawa, Mayumi and Imai, Takashi and 道川 祐市 and 菅 智 and 石川 敦子 and 岩川 眞由美 and 今井 高志},
 issue = {123},
 journal = {BMC Medical Genetics (Online Only URL:http://www.biomedcentral.com/bmcmedgenet)},
 month = {Aug},
 note = {Background
The response of normal tissues in cancer patients undergoing radiotherapy varies, possibly due to genetic differences underlying variation in radiosensitivity.
\nMethods
Cancer patients (n = 360) were selected retrospectively from the RadGenomics project. Adverse effects within 3 months of radiotherapy completion were graded using the National Cancer Institute Common Toxicity Criteria; high grade group were grade 3 or more (n = 180), low grade group were grade 1 or less (n = 180). Pooled genomic DNA (gDNA) (n = 90 from each group) was screened using 23,244 microsatellites. Markers with different inter-group frequencies (Fisher exact test P < 0.05) were analyzed using the remaining pooled gDNA. Silencing RNA treatment was performed in cultured normal human skin fibroblasts.
\nResults
Forty-seven markers had positive association values; including one in the SEMA3A promoter region (P = 1.24 x10-5). SEMA3A knockdown enhanced radiation resistance.
\nConclusions
This study identified 47 putative radiosensitivity markers, and suggested a role for SEMA3A in radiosensitivity.},
 pages = {123-1--123-11},
 title = {Genome wide screen identifies microsatellite markers associated with acute adverse effects following radiotherapy in cancer patients},
 volume = {11},
 year = {2010}
}