@article{oai:repo.qst.go.jp:00047631, author = {Ogawa, Yukihiro and Ueno, Megumi and Sekine-Suzuki, Emiko and Nakanishi, Ikuo and Matsumoto, Ken-ichiro and Fujisaki, Shingo and 小川 幸大 and 上野 恵美 and 関根 絵美子 and 中西 郁夫 and 松本 謙一郎}, issue = {3}, journal = {Journal of Clinical Biochemistry and Nutrition}, month = {Nov}, note = {The aim of this experiment is to measure in vivo generation of melanin-derived radicals non-invasively, as a quantifiable index of radio-biological effect. Melanin-derived radicals in a living intact mouse tail tip were non-invasively measured in very simple way using an X-band electron paramagnetic resonance spectrometer. Colored mouse strains, C57BL/6NCr, BDF1, and C3H/He, has clear EPR signal corresponding to melanin-derived radicals in the tail tip; however, albino mouse strains, BALB/cCr, ddY, ICR, has no EPR signals. An X-ray fraction of 2 Gy/day (1 Gy/min) was repeatedly irradiated to a C3H/He mouse tail skin every Monday to Friday for 4 weeks. In comparison to before starting irradiation, the C3H/He mouse tail skin became darker, like a suntan. The melanin-derived radicals in C3H/He mouse tail skin was increased in association with X-ray fractions. Melanin-derived radicals in mouse tail skin can be readily and chronologically measurable by using X-band EPR spectrometer, and can be a marker for a radiobiological effect in the skin.}, pages = {160--164}, title = {Non-invasive Measurement of Melanin-Derived Radicals in Living Mouse Tail Using X-band EPR}, volume = {59}, year = {2016} }