@article{oai:repo.qst.go.jp:00047622,
 author = {勝部, 孝則 and 王, 冰 and 田中, 薫 and 二宮, 康晴 and Ｇｕｉｌｌａｕｍｅ, Ｖａｒｅｓ and 川越, 大輝 and 塩見, 尚子 and 久保田, 善久 and ＬＩＵ, Ｑｉａｎｇ and 森田, 明典 and 中島, 徹夫 and 根井, 充 and 勝部 孝則 and 王 冰 and 田中 薫 and 二宮 康晴 and Ｇｕｉｌｌａｕｍｅ Ｖａｒｅｓ and 川越 大輝 and 塩見 尚子 and 久保田 善久 and ＬＩＵ Ｑｉａｎｇ and 中島 徹夫 and 根井 充},
 journal = {Mutation Research/Genetic Toxicology and Environmental Mutagenesis},
 month = {Jan},
 note = {Both ionizing radiation (IR) and psychological stress (PS) cause detrimental effects on humans. A recent study showed that chronic restraint-induced PS (CRIPS) diminished the functions of Trp53 and enhanced radiocarcinogenesis in Trp53-heterozygous (Trp53+/-) mice. These findings had a marked impact on the academic field as well as the general public, particularly among residents living in areas radioactively contaminated by nuclear accidents. In an attempt to elucidate the modifying effects of CRIPS on radiation-induced health consequences in Trp53 wild-type (Trp53+/+) animals, investigations involving multidisciplinary analyses were performed. We herein demonstrated that CRIPS induced changes in the frequency of IR-induced chromosomal aberrations (CAs) in splenocytes. Five-week-old male Trp53+/+ C57BL/6J mice were restrained for 6 hours per day for 28 consecutive days, and total body irradiation (TBI) at a dose of 4 Gy was performed on the 8th day. Metaphase chromosome spreads prepared from splenocytes at the end of the 28-day restraint regimen were painted with fluorescence in situ hybridization (FISH) probes for chromosomes 1, 2, and 3. The results obtained showed that CRIPS alone did not induce CAs, while TBI caused significant increases in CAs, mostly translocations. Translocations appeared at a lower frequency in mice exposed to TBI plus CRIPS than in those exposed to TBI alone. No significant differences were observed in the frequencies of the other types of CAs (insertions, dicentrics, and fragments) visualized with FISH between these experimental groups (TBI+CRIPS vs. TBI). These results suggest that CRIPS does not appear to synergize with the clastogenicity of IR.},
 pages = {18--26},
 title = {Effects of chronic restraint-induced stress on radiation-induced chromosomal aberrations in mouse splenocytes},
 volume = {813},
 year = {2017}
}