{"created":"2023-05-15T14:36:57.278448+00:00","id":47621,"links":{},"metadata":{"_buckets":{"deposit":"5a5b49b7-2153-4412-ac91-bd99061425e2"},"_deposit":{"created_by":1,"id":"47621","owners":[1],"pid":{"revision_id":0,"type":"depid","value":"47621"},"status":"published"},"_oai":{"id":"oai:repo.qst.go.jp:00047621","sets":["1"]},"author_link":["477390","477391","477389","477387","477386","477388","477392"],"item_8_biblio_info_7":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2016-11","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"1","bibliographicPageEnd":"13","bibliographicPageStart":"7","bibliographicVolumeNumber":"30","bibliographic_titles":[{"bibliographic_title":"Protein Engineering, Design & Selection"}]}]},"item_8_description_5":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"ウミシイタケ由来ルシフェラーゼ(RLuc)は発光物質（ルシフェリン）が光を放つ化学反応を触媒する酵素であり、遺伝子制御および機能研究や創薬スクリーニングなどのレポーターとして広く利用されている。しかし、野生型RLucは不安定であり、～35℃付近から変性してしまうなどの欠点があった。そこで本研究では、RLucにランダムな変異導入を行い、野生型よりも熱安定性が高いRLucの創製を試みた。本研究の結果、野生型よりも熱変性温度が10℃程度高いN264SS287P変異型RLucの創製に成功した。また、分子モデリングにより、N264SおよびS287P変異導入がRLucの熱安定性を向上する機構を解明することができた。本成果により、これまで熱安定性などの問題から適用が諦められていた基礎・応用研究分野におけるルシフェラーゼの利用範囲の拡大が期待される。","subitem_description_type":"Abstract"}]},"item_8_publisher_8":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"  OXFORD UNIVERSITY PRESS"}]},"item_8_relation_14":{"attribute_name":"DOI","attribute_value_mlt":[{"subitem_relation_type_id":{"subitem_relation_type_id_text":"10.1093/protein/gzw056 ","subitem_relation_type_select":"DOI"}}]},"item_access_right":{"attribute_name":"アクセス権","attribute_value_mlt":[{"subitem_access_right":"metadata only access","subitem_access_right_uri":"http://purl.org/coar/access_right/c_14cb"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"新井, 栄揮"}],"nameIdentifiers":[{"nameIdentifier":"477386","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"石橋, 松二郎(鹿児島大学農学部)"}],"nameIdentifiers":[{"nameIdentifier":"477387","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"重久, 愛(鹿児島大学農学部)"}],"nameIdentifiers":[{"nameIdentifier":"477388","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"天羽, 紀絵(鹿児島大学農学部)"}],"nameIdentifiers":[{"nameIdentifier":"477389","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"東, 千里(鹿児島大学農学部)"}],"nameIdentifiers":[{"nameIdentifier":"477390","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"al., et"}],"nameIdentifiers":[{"nameIdentifier":"477391","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"新井 栄揮","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"477392","nameIdentifierScheme":"WEKO"}]}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"jpn"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"Stabilization of luciferase from Renilla reniformis using random mutations","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Stabilization of luciferase from Renilla reniformis using random mutations"}]},"item_type_id":"8","owner":"1","path":["1"],"pubdate":{"attribute_name":"公開日","attribute_value":"2017-01-30"},"publish_date":"2017-01-30","publish_status":"0","recid":"47621","relation_version_is_last":true,"title":["Stabilization of luciferase from Renilla reniformis using random mutations"],"weko_creator_id":"1","weko_shared_id":-1},"updated":"2023-05-15T23:37:31.518904+00:00"}