{"created":"2023-05-15T14:36:52.321833+00:00","id":47508,"links":{},"metadata":{"_buckets":{"deposit":"134b89e4-cb3c-456a-8798-b1c0d11b2722"},"_deposit":{"created_by":1,"id":"47508","owners":[1],"pid":{"revision_id":0,"type":"depid","value":"47508"},"status":"published"},"_oai":{"id":"oai:repo.qst.go.jp:00047508","sets":["1"]},"author_link":["476040","476047","476048","476043","476038","476036","476041","476042","476049","476050","476046","476045","476044","476039","476037"],"item_8_biblio_info_7":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2015-08","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"166","bibliographicVolumeNumber":"14","bibliographic_titles":[{"bibliographic_title":"Molecular Cancer (Online Only URL:http://www.molecular-cancer.com/)"}]}]},"item_8_description_5":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"Aims: Although a relatively small proportion of all breast cancer (BC), triple negative (TN) BC is responsible for a relatively large proportion of BC deaths because of its worse clinical outcome. To investigate whether a carbon ion beam alone or in combination with cisplatin has a beneficial effect compared to X-rays, we target triple negative (TN) breast cancer stem-like cells (CSCs). \nMethods: Human breast CSCs sorted from MDA-MB-231 cells were treated with a carbon ion beam or X-ray irradiation alone or in combination with cisplatin (CDDP), and then colony, spheroid and tumor formation assays, RT-PCR Array analysis, and immunofluorescence γH2AX foci assay were performed.\nResults: The colony, spheroid formation, and tumorigenicity assays confirmed that CD44+/CD24- and ESA+/CD24- cells have CSC properties in MDA-MB-231 and MDA-MB-453 cells, respectively. The proportion of CSCs was more enriched after CDDP combination with either X-ray or carbon ion beam, however carbon ion beam combined with CDDP significantly suppressed colony and spheroid formation and more significantly inhibited cell cycle progression (sub-G1 arrest) compared to X-ray combined with CDDP or carbon ion beam alone. RT-PCR Array analysis showed that carbon ion beam combined with CDDP significantly induced apoptosis-related Cytochrome c, almost completely eliminated expression of the CSC markers CD44 and ESA, and significantly inhibited angiogenesis, and metastasis-related HIF1α and CD26 compared to carbon ion beam alone, X-ray alone, or X-ray combined with CDDP. The immunofluorescence assay showed that not only the number but also the size of γH2AX foci in CSCs were larger 24 h after carbon ion beam combined with CDDP compared to those of X-ray alone and X-ray combined with CDDP. \nConclusions: Carbon ion beam combined with CDDP has superior potential to kill TN breast CSCs with irreparable severe DNA damage and enhanced apoptosis.","subitem_description_type":"Abstract"}]},"item_8_publisher_8":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"BioMed Central Limited"}]},"item_8_relation_14":{"attribute_name":"DOI","attribute_value_mlt":[{"subitem_relation_type_id":{"subitem_relation_type_id_text":"10.1186/s12943-015-0429-7.","subitem_relation_type_select":"DOI"}}]},"item_8_source_id_9":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"1476-4598","subitem_source_identifier_type":"ISSN"}]},"item_access_right":{"attribute_name":"アクセス権","attribute_value_mlt":[{"subitem_access_right":"metadata only access","subitem_access_right_uri":"http://purl.org/coar/access_right/c_14cb"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Sai, Sei"}],"nameIdentifiers":[{"nameIdentifier":"476036","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Vares, Guillaume"}],"nameIdentifiers":[{"nameIdentifier":"476037","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Ho, Kim Eun"}],"nameIdentifiers":[{"nameIdentifier":"476038","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Karasawa, Kumiko"}],"nameIdentifiers":[{"nameIdentifier":"476039","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Bing, Wang"}],"nameIdentifiers":[{"nameIdentifier":"476040","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Nenoi, Mitsuru"}],"nameIdentifiers":[{"nameIdentifier":"476041","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Horimoto, Yoshiya"}],"nameIdentifiers":[{"nameIdentifier":"476042","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"al, et"}],"nameIdentifiers":[{"nameIdentifier":"476043","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"崔 星","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"476044","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Ｇｕｉｌｌａｕｍｅ Ｖａｒｅｓ","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"476045","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Ｋｉｍ Ｅｕｎ Ｈｏ","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"476046","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"唐澤 久美子","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"476047","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"王 冰","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"476048","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"根井 充","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"476049","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"堀本 義哉","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"476050","nameIdentifierScheme":"WEKO"}]}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"Carbon Ion Beam Combined with Cisplatin Effectively Disrupts Triple Negative Breast Cancer Stem-Like Cells In Vitro","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Carbon Ion Beam Combined with Cisplatin Effectively Disrupts Triple Negative Breast Cancer Stem-Like Cells In Vitro"}]},"item_type_id":"8","owner":"1","path":["1"],"pubdate":{"attribute_name":"公開日","attribute_value":"2016-08-25"},"publish_date":"2016-08-25","publish_status":"0","recid":"47508","relation_version_is_last":true,"title":["Carbon Ion Beam Combined with Cisplatin Effectively Disrupts Triple Negative Breast Cancer Stem-Like Cells In Vitro"],"weko_creator_id":"1","weko_shared_id":-1},"updated":"2023-05-15T23:38:58.112115+00:00"}