{"created":"2023-05-15T14:36:47.224285+00:00","id":47396,"links":{},"metadata":{"_buckets":{"deposit":"7994ca80-d37c-417e-a7ac-c3bdbba98e90"},"_deposit":{"created_by":1,"id":"47396","owners":[1],"pid":{"revision_id":0,"type":"depid","value":"47396"},"status":"published"},"_oai":{"id":"oai:repo.qst.go.jp:00047396","sets":["1"]},"author_link":["474514","474520","474512","474516","474518","474517","474519","474511","474515","474510","474513"],"item_8_biblio_info_7":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2015-04","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"4","bibliographicPageEnd":"638","bibliographicPageStart":"633","bibliographicVolumeNumber":"56","bibliographic_titles":[{"bibliographic_title":"Journal of radiation research"}]}]},"item_8_description_5":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"While it has been expected that X-ray laser will be widely applied to biomedical studies, this has not been achieved to date and its biological effects such as DNA damage have not been evaluated. As a first step for its biological application, we developed a culture cell irradiation system, particularly designed for a plasma-driven soft X-ray laser pulse, to investigate whether the soft X-ray laser is able to induce DNA double strand breaks (DSBs) in living cells or not. The human adenocarcimona cell line A549 was irradiated with the soft X-ray laser at a photon energy of 89 eV and the repair focus formation of the DSBs was assessed by immunofluorescence staining with antiphosphorylated DNA-PKcs (p-DNA-PKcs), ATM (p-ATM) and γ-H2AX antibody. The p-DNA-PKcs, ATM, and γ-H2AX foci were clearly identified after soft X-ray laser irradiation. Furthermore, the increase in the X-ray laser shot number, even from a single shot, results in the increase in p-DNA-PKcs foci. These results are the first evidence that the 89 eV soft X-ray laser is able to induce DSB in living cells. Our study demonstrated that this irradiation system is a useful tool for investigating the radiobiological effect of soft X-ray laser.","subitem_description_type":"Abstract"}]},"item_8_publisher_8":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"Oxford University Press"}]},"item_8_relation_13":{"attribute_name":"PubMed番号","attribute_value_mlt":[{"subitem_relation_type_id":{"subitem_relation_type_id_text":"25862698","subitem_relation_type_select":"PMID"}}]},"item_8_relation_14":{"attribute_name":"DOI","attribute_value_mlt":[{"subitem_relation_type_id":{"subitem_relation_type_id_text":"10.1093/jrr/rrv015","subitem_relation_type_select":"DOI"}}]},"item_8_relation_17":{"attribute_name":"関連サイト","attribute_value_mlt":[{"subitem_relation_name":[{"subitem_relation_name_text":"http://jrr.oxfordjournals.org/content/early/2015/04/09/jrr.rrv015.full"}],"subitem_relation_type_id":{"subitem_relation_type_id_text":"http://jrr.oxfordjournals.org/content/early/2015/04/09/jrr.rrv015.full","subitem_relation_type_select":"URI"}}]},"item_8_source_id_9":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"0449-3060","subitem_source_identifier_type":"ISSN"}]},"item_access_right":{"attribute_name":"アクセス権","attribute_value_mlt":[{"subitem_access_right":"metadata only access","subitem_access_right_uri":"http://purl.org/coar/access_right/c_14cb"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Sato, Katsutoshi"}],"nameIdentifiers":[{"nameIdentifier":"474510","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Nishikino, Masaharu"}],"nameIdentifiers":[{"nameIdentifier":"474511","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Kawachi, Tetsuya"}],"nameIdentifiers":[{"nameIdentifier":"474512","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Shimokawa, Takashi"}],"nameIdentifiers":[{"nameIdentifier":"474513","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Imai, Takashi"}],"nameIdentifiers":[{"nameIdentifier":"474514","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Teshima, Teruki"}],"nameIdentifiers":[{"nameIdentifier":"474515","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Nishimura, Hiroaki"}],"nameIdentifiers":[{"nameIdentifier":"474516","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Kando, Masaki"}],"nameIdentifiers":[{"nameIdentifier":"474517","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"佐藤 克俊","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"474518","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"下川 卓志","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"474519","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"今井 高志","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"474520","nameIdentifierScheme":"WEKO"}]}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"A laser-plasma-produced soft X-ray laser at 89 eV generates DNA double-strand breaks in human cancer cells.","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"A laser-plasma-produced soft X-ray laser at 89 eV generates DNA double-strand breaks in human cancer cells."}]},"item_type_id":"8","owner":"1","path":["1"],"pubdate":{"attribute_name":"公開日","attribute_value":"2016-05-18"},"publish_date":"2016-05-18","publish_status":"0","recid":"47396","relation_version_is_last":true,"title":["A laser-plasma-produced soft X-ray laser at 89 eV generates DNA double-strand breaks in human cancer cells."],"weko_creator_id":"1","weko_shared_id":-1},"updated":"2023-05-15T23:40:21.393673+00:00"}