{"created":"2023-05-15T14:36:41.351041+00:00","id":47263,"links":{},"metadata":{"_buckets":{"deposit":"b3ffa61e-90db-4bc3-bb6d-4d9aa8697eba"},"_deposit":{"created_by":1,"id":"47263","owners":[1],"pid":{"revision_id":0,"type":"depid","value":"47263"},"status":"published"},"_oai":{"id":"oai:repo.qst.go.jp:00047263","sets":["1"]},"author_link":["472715","472708","472716","472712","472711","472707","472713","472710","472709","472714"],"item_8_biblio_info_7":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2011-08","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"6","bibliographicPageEnd":"1732","bibliographicPageStart":"1719","bibliographicVolumeNumber":"6","bibliographic_titles":[{"bibliographic_title":"International Journal of Nanomedicine"}]}]},"item_8_description_5":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"Background: One of the most attractive properties of quantum dots is their potential to extend\nthe opportunities for fluorescent and multimodal imaging in vivo. The aim of the present study\nwas to clarify whether the composition and structure of organic coating of nanoparticles are\ncrucial for their application in vivo.\nMethods: We compared quantum dots coated with non-crosslinked amino-\nfunctionalized polyamidoamine\n(PAMAM) dendrimers, quantum dots encapsulated in crosslinked carboxyl-\nfunctionalized\nPAMAM dendrimers, and silica-shelled amino-functionalized quantum dots. A multimodal\nfluorescent and paramagnetic quantum dot probe was also developed and analyzed. The probes\nwere applied intravenously in anesthetized animals for visualization of brain vasculature using\ntwo-photon excited fluorescent microscopy and visualization of tumors using fluorescent IVIS®\nimaging (Caliper Life Sciences, Hopkinton, MA) and magnetic resonance imaging.\nResults: Quantum dots coated with non-crosslinked dendrimers were cytotoxic. They induced side\neffects in vivo, including vasodilatation with a decrease in mean arterial blood pressure and heart\nrate. The quantum dots penetrated the vessels, which caused the quality of fluorescent imaging to\ndeteriorate. Quantum dots encapsulated in crosslinked dendrimers had low cytotoxicity and were\nbiocompatible. In concentrations ?0.3 nmol quantum dots/kg bodyweight, these nanoparticles\ndid not affect blood pressure and heart rate, and did not induce vasodilatation or vasoconstriction.\nPEGylation (PEG [polyethylene glycol]) was an indispensable step in development of a\nquantum dot probe for in vivo imaging, based on silica-shelled quantum dots. The non-PEGylated\nsilica-shelled quantum dots possessed low colloidal stability in high-salt physiological fluids,\naccompanied by rapid aggregation in vivo. The conjugation of silica-shelled quantum dots with\nPEG1100 increased their stability and half-life in the circulation without significant enhancement\nof their size. In concentrations ?2.5 nmol/kg bodyweight, these quantum dots did not affect the\nmain physiological variables. It was possible to visualize capillaries, which makes this quantum\ndot probe appropriate for investigation of mediators of vasoconstriction, vasodilatation, and brain\ncirculation in intact animals in vivo. The multimodal silica-shelled quantum dots allowed visualization\nof tumor tissue in an early stage of its development, using magnetic resonance imaging.\nConclusion: The present study shows that the type and structure of organic/bioorganic shells of\nquantum dots determine their biocompatibility and are crucial for their application in imaging\nin vivo, due to the effects of the shell on the following properties: colloidal stability, solubility\nin physiological fluids, influence of the basic physiological parameters, and cytotoxicity.","subitem_description_type":"Abstract"}]},"item_8_relation_14":{"attribute_name":"DOI","attribute_value_mlt":[{"subitem_relation_type_id":{"subitem_relation_type_id_text":"10.2147/ijn.s17995","subitem_relation_type_select":"DOI"}}]},"item_8_source_id_9":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"1176-9114","subitem_source_identifier_type":"ISSN"}]},"item_access_right":{"attribute_name":"アクセス権","attribute_value_mlt":[{"subitem_access_right":"metadata only access","subitem_access_right_uri":"http://purl.org/coar/access_right/c_14cb"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Bakalova-Zheleva, Rumiana"}],"nameIdentifiers":[{"nameIdentifier":"472707","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Zhelev, Zhivko"}],"nameIdentifiers":[{"nameIdentifier":"472708","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Kokuryo, Daisuke"}],"nameIdentifiers":[{"nameIdentifier":"472709","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Aoki, Ichio"}],"nameIdentifiers":[{"nameIdentifier":"472710","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Saga, Tsuneo"}],"nameIdentifiers":[{"nameIdentifier":"472711","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"バカロバ ルミアナ","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"472712","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Ｚｈｅｌｅｖ Ｚｈｉｖｋｏ","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"472713","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"國領 大介","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"472714","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"青木 伊知男","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"472715","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"佐賀 恒夫","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"472716","nameIdentifierScheme":"WEKO"}]}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"Chemical nature and structure of organic coating of quantum dots is crucial for their application in imaging diagnostics.","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Chemical nature and structure of organic coating of quantum dots is crucial for their application in imaging diagnostics."}]},"item_type_id":"8","owner":"1","path":["1"],"pubdate":{"attribute_name":"公開日","attribute_value":"2015-08-19"},"publish_date":"2015-08-19","publish_status":"0","recid":"47263","relation_version_is_last":true,"title":["Chemical nature and structure of organic coating of quantum dots is crucial for their application in imaging diagnostics."],"weko_creator_id":"1","weko_shared_id":-1},"updated":"2023-05-15T23:41:57.193001+00:00"}