{"created":"2023-05-15T14:36:31.746425+00:00","id":47046,"links":{},"metadata":{"_buckets":{"deposit":"19c88133-115f-4378-827e-3da0ebe889c2"},"_deposit":{"created_by":1,"id":"47046","owners":[1],"pid":{"revision_id":0,"type":"depid","value":"47046"},"status":"published"},"_oai":{"id":"oai:repo.qst.go.jp:00047046","sets":["1"]},"author_link":["469781","469772","469777","469769","469780","469770","469778","469773","469776","469771","469775","469779","469774"],"item_8_biblio_info_7":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2015-03","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"3","bibliographicPageEnd":"376","bibliographicPageStart":"371","bibliographicVolumeNumber":"108","bibliographic_titles":[{"bibliographic_title":"Health Physics"}]}]},"item_8_description_5":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"The dicentric chromosome assay (DCA) has been regarded\nas the gold standard of radiation biodosimetry. The assay,\nhowever, requires a 2-d peripheral blood lymphocyte culture before\nstarting metaphase chromosome analyses to estimate biological\ndoses. Other biological assays also have drawbacks with respect\nto the time needed to obtain dose estimates for rapid decision on\nthe correct line of medical treatment. Therefore, alternative technologies\nthat suit requirements for triage biodosimetry are needed.\nRadiation-induced DNA double strand breaks in G0 lymphocytes\ncan be detected as interphase chromosome aberrations by the\ncell fusion-mediated premature chromosome condensation (PCC)\nmethod. The method, in combination with fluorescence in situ hybridization\n(FISH) techniques, has been proposed in early studies\nas a powerful tool for obtaining biological dose estimates without\n2-d lymphocyte culture procedures. The present work assesses the\napplicability of FISH-based PCC techniques using pan-centromeric\nand telomeric peptide nucleic acid (PNA) probes in triage mode\nbiodosimetry and demonstrates that an improved rapid procedure\nof the prematurely condensed dicentric chromosome (PCDC) assay\nhas the potential for evaluating exposed radiation doses in as\nshort as 6 h after the collection of peripheral blood specimens.","subitem_description_type":"Abstract"}]},"item_8_publisher_8":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"Wolters Kluwer"}]},"item_8_source_id_9":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"0017-9078","subitem_source_identifier_type":"ISSN"}]},"item_access_right":{"attribute_name":"アクセス権","attribute_value_mlt":[{"subitem_access_right":"metadata only access","subitem_access_right_uri":"http://purl.org/coar/access_right/c_14cb"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"數藤, 由美子"}],"nameIdentifiers":[{"nameIdentifier":"469769","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"後藤, 孝也"}],"nameIdentifiers":[{"nameIdentifier":"469770","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"野田, 隆司"}],"nameIdentifiers":[{"nameIdentifier":"469771","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"穐山, 美穂"}],"nameIdentifiers":[{"nameIdentifier":"469772","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"大脇, 真紀子"}],"nameIdentifiers":[{"nameIdentifier":"469773","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Darroudi, Firouz"}],"nameIdentifiers":[{"nameIdentifier":"469774","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"平井, 百樹"}],"nameIdentifiers":[{"nameIdentifier":"469775","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"數藤 由美子","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"469776","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"後藤 孝也","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"469777","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"野田 隆司","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"469778","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"穐山 美穂","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"469779","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"大脇 真紀子","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"469780","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"平井 百樹","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"469781","nameIdentifierScheme":"WEKO"}]}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"Assessing the applicability of FISH-based prematurely condensed dicentric chromosome assay in triage biodosimetry","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Assessing the applicability of FISH-based prematurely condensed dicentric chromosome assay in triage biodosimetry"}]},"item_type_id":"8","owner":"1","path":["1"],"pubdate":{"attribute_name":"公開日","attribute_value":"2015-03-19"},"publish_date":"2015-03-19","publish_status":"0","recid":"47046","relation_version_is_last":true,"title":["Assessing the applicability of FISH-based prematurely condensed dicentric chromosome assay in triage biodosimetry"],"weko_creator_id":"1","weko_shared_id":-1},"updated":"2023-05-15T23:44:32.975388+00:00"}