@article{oai:repo.qst.go.jp:00047046,
 author = {數藤, 由美子 and 後藤, 孝也 and 野田, 隆司 and 穐山, 美穂 and 大脇, 真紀子 and Darroudi, Firouz and 平井, 百樹 and 數藤 由美子 and 後藤 孝也 and 野田 隆司 and 穐山 美穂 and 大脇 真紀子 and 平井 百樹},
 issue = {3},
 journal = {Health Physics},
 month = {Mar},
 note = {The dicentric chromosome assay (DCA) has been regarded
as the gold standard of radiation biodosimetry. The assay,
however, requires a 2-d peripheral blood lymphocyte culture before
starting metaphase chromosome analyses to estimate biological
doses. Other biological assays also have drawbacks with respect
to the time needed to obtain dose estimates for rapid decision on
the correct line of medical treatment. Therefore, alternative technologies
that suit requirements for triage biodosimetry are needed.
Radiation-induced DNA double strand breaks in G0 lymphocytes
can be detected as interphase chromosome aberrations by the
cell fusion-mediated premature chromosome condensation (PCC)
method. The method, in combination with fluorescence in situ hybridization
(FISH) techniques, has been proposed in early studies
as a powerful tool for obtaining biological dose estimates without
2-d lymphocyte culture procedures. The present work assesses the
applicability of FISH-based PCC techniques using pan-centromeric
and telomeric peptide nucleic acid (PNA) probes in triage mode
biodosimetry and demonstrates that an improved rapid procedure
of the prematurely condensed dicentric chromosome (PCDC) assay
has the potential for evaluating exposed radiation doses in as
short as 6 h after the collection of peripheral blood specimens.},
 pages = {371--376},
 title = {Assessing the applicability of FISH-based prematurely condensed dicentric chromosome assay in triage biodosimetry},
 volume = {108},
 year = {2015}
}