@article{oai:repo.qst.go.jp:00046996,
 author = {Takahashi, Momoko and Hirakawa, Hirokazu and Yajima, Hirohiko and Izumi-Nakajima, Nakako and Okayasu, Ryuichi and Fujimori, Akira and 平川 博一 and 矢島 浩彦 and 中島 菜花子 and 岡安 隆一 and 藤森 亮},
 issue = {12},
 journal = {International journal of radiation biology},
 month = {Aug},
 note = {Purpose: To obtain human glioblastoma cells A172 expressing stem cell-related protein and comparison of radiosensitivity in these cells with X-rays and carbon beam. Methods: Human monolayer-type A172 glioblastoma cells were maintained in normal medium with 10% bovine serum. In order to obtain sphere-type A172 cells the medium was replaced with serum-free medium supplemented with growth factors. Both types of A172 cells were irradiated with either X-rays or carbon ion beams and their radiosensitivity was evaluated. Results: Serum-free medium induced expression of stem cell-related proteins in A172 cells along with the neurosphere-like appearance. These sphere-type cells were found resistant to both X-rays and carbon ion beams. Phosphorylation of histone H2A family member X persisted for a longer period in the cells exposed to carbon ion beams than in those exposed to X-rays and it disappeared quicker in the sphere type than in the monolayer type. Relative radioresistance of the sphere type cells was smaller for carbon ion beams than for X-rays. Conclusions: We demonstrated that glioblastoma A172 cells with induced stem cell-related proteins turned resistant to irradiation. Accelerated heavy ion particles may have advantage over X-rays in overcoming the tumor resistance due to cell stemness.},
 pages = {1125--1132},
 title = {Carbon ion beam is more effective to induce cell death in sphere-type A172 human glioblastoma cells compared with X-rays.},
 volume = {90},
 year = {2014}
}