@article{oai:repo.qst.go.jp:00046989,
 author = {Sugashi, Takuma and Yoshihara, Kouichi and Kawaguchi, Hiroshi and Takuwa, Hiroyuki and Ito, Hiroshi and Kanno, Iwao and Yamada, Yukio and Masamoto, Kazuto and 須貸 拓馬 and 吉原 光一 and 川口 拓之 and 田桑 弘之 and 伊藤 浩 and 菅野 巖 and 正本 和人},
 journal = {Advances in experimental medicine and biology},
 month = {Apr},
 note = {The present study was aimed to characterize 3-dimensional (3D) morphology of the cortical microvasculature (e.g., penetrating artery and emerging vein), using two-photon microscopy and automated analysis for their cross-sectional diameters and branching positions in the mouse cortex. We observed that both artery and vein had variable cross-sectional diameters across cortical depths. The mean diameter was similar for both artery (17 ± 5 μm) and vein (15 ± 5 μm), and there were no detectable differences over depths of 50-400 μm. On the other hand, the number of branches was slightly increased up to 400-μm depth for both the artery and vein. The mean number of branches per 0.1 mm vessel length was 1.7 ± 1.2 and 3.8 ± 1.6 for the artery and vein, respectively. This method allows for quantification of the large volume data of microvascular images captured with two-photon microscopy. This will contribute to the morphometric analysis of the cortical microvasculature in functioning brains.},
 pages = {209--215},
 title = {Automated image analysis for diameters and branching points of cerebral penetrating arteries and veins captured with two-photon microscopy.},
 volume = {812},
 year = {2014}
}