@article{oai:repo.qst.go.jp:00046988,
 author = {Murata, Rei and Takada, Yuki and Takuwa, Hiroyuki and Kawaguchi, Hiroshi and Ito, Hiroshi and Kanno, Iwao and Tottori, Naotomo and Yamada, Yukio and Tomita, Yutaka and Itoh, Yoshiaki and Suzuki, Norihiro and Yamada, Katsuya and Masamoto, Kazuto and 村田 澪 and 高田 優季 and 田桑 弘之 and 川口 拓之 and 伊藤 浩 and 菅野 巖 and 鳥取 直友 and 冨田 裕 and 正本 和人},
 journal = {Advances in experimental medicine and biology},
 month = {Apr},
 note = {The present study examined glucose transfer in the cellular scale of mouse brain microvasculature in vivo using two-photon microscopy and fluorescent glucose analogue (2-NBDG). The 2-NBDG was intravenously injected (0.04 mL/min) in the anesthetized Tie2-GFP mice in which the vascular endothelium expressed fluorescent protein. Time-lapse imaging was conducted on the cortical parenchyma, while the time-intensity change of the injected 2-NBDG was analysed in respective vascular compartments (artery, capillary, and vein). We observed that 2-NBDG signal increased monotonically in the vasculature during the period of the injection, and rapidly declined following its cessation. In tissue compartment, however, the signal intensity gradually increased even after cessation of the injection. Spatiotemporal analysis of the 2-NBDG intensity over the cross-sections of the vessels further showed distinct change of the 2-NBDG intensity across the vessel wall (endothelium), which may represents a regulation site of tissue glucose influx.},
 pages = {241--246},
 title = {Vessel specific imaging of glucose transfer with fluorescent glucose analogue in anesthetized mouse cortex.},
 volume = {812},
 year = {2014}
}