{"created":"2023-05-15T14:36:26.759807+00:00","id":46931,"links":{},"metadata":{"_buckets":{"deposit":"fb3c08f9-106d-4567-8fd9-68ece42609d0"},"_deposit":{"created_by":1,"id":"46931","owners":[1],"pid":{"revision_id":0,"type":"depid","value":"46931"},"status":"published"},"_oai":{"id":"oai:repo.qst.go.jp:00046931","sets":["1"]},"author_link":["468364","468362","468365","468363"],"item_8_biblio_info_7":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2013-06","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"6","bibliographicPageStart":"e66801","bibliographicVolumeNumber":"8","bibliographic_titles":[{"bibliographic_title":"PLoS ONE (Online only:URL:http://www.plosone.org)"}]}]},"item_8_description_5":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"The breast cancer 1 (BRCA1) protein is a tumor suppressor playing roles in DNA repair and cell cycle regulation. Studies of DNA repair functions of BRCA1 have focused on double-strand break (DSB) repair pathways and have recently included base excision repair (BER). However, the function of BRCA1 in BER is not well defined. Here, we examined a BRCA1 role in BER, first in relation to alkylating agent (MMS) treatment of cells and the BER enzyme DNA polymerase b (pol b). MMS treatment of BRCA1 negative human ovarian and chicken DT40 cells revealed hypersensitivity, and the combined gene deletion of BRCA1 and pol b in DT40 cells was consistent with these factors acting in the same repair pathway, possibly BER. Using cell extracts and purified proteins, BRCA1 and pol b were found to interact in immunoprecipitation assays, yet in vivo and in vitro assays for a BER role of BRCA1 were negative. An alternate approach with the human cells of immunofluorescence imaging and laser-induced DNA damage revealed negligible BRCA1 recruitment during the first 60 s after irradiation, the period typical of recruitment of pol b and other BER factors. Instead, 15 min after irradiation, BRCA1 recruitment was strong and there was c- H2AX co-localization, consistent with DSBs and repair. The rapid recruitment of pol b was similar in BRCA1 positive and negative cells. However, a fraction of pol b initially recruited remained associated with damage sites much longer in BRCA1 positive than negative cells. Interestingly, pol b expression was required for BRCA1 recruitment, suggesting a partnership between these repair factors in DSB repair.","subitem_description_type":"Abstract"}]},"item_8_relation_14":{"attribute_name":"DOI","attribute_value_mlt":[{"subitem_relation_type_id":{"subitem_relation_type_id_text":"10.1371/journal.pone.0066801","subitem_relation_type_select":"DOI"}}]},"item_8_source_id_9":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"1932-6203","subitem_source_identifier_type":"ISSN"}]},"item_access_right":{"attribute_name":"アクセス権","attribute_value_mlt":[{"subitem_access_right":"metadata only access","subitem_access_right_uri":"http://purl.org/coar/access_right/c_14cb"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Masaoka, Aya"}],"nameIdentifiers":[{"nameIdentifier":"468362","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Wilson, Samuel"}],"nameIdentifiers":[{"nameIdentifier":"468363","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"et.al"}],"nameIdentifiers":[{"nameIdentifier":"468364","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"正岡 綾","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"468365","nameIdentifierScheme":"WEKO"}]}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"Interaction between DNA Polymerase b and BRCA1","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Interaction between DNA Polymerase b and BRCA1"}]},"item_type_id":"8","owner":"1","path":["1"],"pubdate":{"attribute_name":"公開日","attribute_value":"2014-11-13"},"publish_date":"2014-11-13","publish_status":"0","recid":"46931","relation_version_is_last":true,"title":["Interaction between DNA Polymerase b and BRCA1"],"weko_creator_id":"1","weko_shared_id":-1},"updated":"2023-05-15T23:45:54.403382+00:00"}