{"created":"2023-05-15T14:36:26.172831+00:00","id":46918,"links":{},"metadata":{"_buckets":{"deposit":"a69289fc-3819-4afb-b89d-1bfbc28ed234"},"_deposit":{"created_by":1,"id":"46918","owners":[1],"pid":{"revision_id":0,"type":"depid","value":"46918"},"status":"published"},"_oai":{"id":"oai:repo.qst.go.jp:00046918","sets":["1"]},"author_link":["468203","468214","468209","468202","468207","468201","468205","468204","468212","468210","468211","468213","468208","468200","468206"],"item_8_biblio_info_7":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2013-11","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"8","bibliographicPageEnd":"669","bibliographicPageStart":"661","bibliographicVolumeNumber":"36","bibliographic_titles":[{"bibliographic_title":"Brain & development"}]}]},"item_8_description_5":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"Aims: A number of ASPM mutations have been detected in primary microcephaly patients. In order to evaluate the function of ASPM in brain development, we generated model animals of human autosomal recessive primary microcephaly-5 (MCPH5). Methods: In the Aspm knock-out mice, the exon 2-3 of the Aspm gene was encompassed by a pair of loxP signals so that cre-recombinase activity switched the allele from wild-type to null zygotes as frequently, as expected from the Mendelian inheritance. We precisely analyzed the brains of adults and fetuses using immunohistochemistry and morphometry. Results: The adult brains of the Aspm(-/-) mice were smaller, especially in the cerebrum. In the barrel field of the somatosensory cortex, layer I was significantly thicker, whereas layer VI was significantly thinner in Aspm(-/-) mice, compared with Aspm(+/+) mice. The total number of cells and the thickness of the cortical plate at embryonic day 16.5 was significantly decreased in Aspm(-/-) mice, compared with Aspm(+/+) mice. Furthermore, the expression of transcription factors, such as Tbr1 and Satb2, was significantly increased in the subplate of the Aspm(-/-) mice. Conclusions: The results suggested that Aspm is essential to the proliferation and differentiation of neural stem/progenitor cells. The Aspm gene loss model provided a novel pathogenetic insight into acquired microcephaly, which can be caused by in utero exposure to both known and unknown teratogens.","subitem_description_type":"Abstract"}]},"item_8_publisher_8":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"Elsevier"}]},"item_8_relation_13":{"attribute_name":"PubMed番号","attribute_value_mlt":[{"subitem_relation_type_id":{"subitem_relation_type_id_text":"24220505","subitem_relation_type_select":"PMID"}}]},"item_8_relation_14":{"attribute_name":"DOI","attribute_value_mlt":[{"subitem_relation_type_id":{"subitem_relation_type_id_text":"10.1016/j.braindev.2013.10.006","subitem_relation_type_select":"DOI"}}]},"item_8_source_id_9":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"0387-7604","subitem_source_identifier_type":"ISSN"}]},"item_access_right":{"attribute_name":"アクセス権","attribute_value_mlt":[{"subitem_access_right":"metadata only access","subitem_access_right_uri":"http://purl.org/coar/access_right/c_14cb"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Fujimori, Akira"}],"nameIdentifiers":[{"nameIdentifier":"468200","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Itoh, Kyoko"}],"nameIdentifiers":[{"nameIdentifier":"468201","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Goto, Shoko"}],"nameIdentifiers":[{"nameIdentifier":"468202","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Hirakawa, Hirokazu"}],"nameIdentifiers":[{"nameIdentifier":"468203","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Wang, Bing"}],"nameIdentifiers":[{"nameIdentifier":"468204","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Kokubo, Toshiaki"}],"nameIdentifiers":[{"nameIdentifier":"468205","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Kito, Seiji"}],"nameIdentifiers":[{"nameIdentifier":"468206","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Tsukamoto, Satoshi"}],"nameIdentifiers":[{"nameIdentifier":"468207","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Fushiki, Shinji"}],"nameIdentifiers":[{"nameIdentifier":"468208","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"藤森 亮","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"468209","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"平川 博一","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"468210","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"王 冰","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"468211","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"小久保 年章","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"468212","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"鬼頭 靖司","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"468213","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"塚本 智史","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"468214","nameIdentifierScheme":"WEKO"}]}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"Disruption of Aspm causes microcephaly with abnormal neuronal differentiation.","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Disruption of Aspm causes microcephaly with abnormal neuronal differentiation."}]},"item_type_id":"8","owner":"1","path":["1"],"pubdate":{"attribute_name":"公開日","attribute_value":"2014-11-10"},"publish_date":"2014-11-10","publish_status":"0","recid":"46918","relation_version_is_last":true,"title":["Disruption of Aspm causes microcephaly with abnormal neuronal differentiation."],"weko_creator_id":"1","weko_shared_id":-1},"updated":"2023-05-15T23:46:03.826189+00:00"}