@article{oai:repo.qst.go.jp:00046908,
 author = {Sekiguchi, Yuta and Masamoto, Kazuto and Takuwa, Hiroyuki and Kawaguchi, Hiroshi and Kanno, Iwao and Ito, Hiroshi and Tomita, Yutaka and Itoh, Yoshiaki and Suzuki, Norihiro and Sudo, Ryo and Tanishita, Kazuo and 関口 優太 and 正本 和人 and 田桑 弘之 and 川口 拓之 and 菅野 巖 and 伊藤 浩 and 冨田 裕},
 journal = {Oxygen Transport to Tissue XXXV (Advances in Experimental Medicine and Biology ; v.789)},
 month = {Jul},
 note = {The present study reports a semiautomatic image analysis method for measuring the spatiotemporal dynamics of the vessel dilation that was fluorescently imaged with either confocal or two-photon microscope. With this method, arterial dilation induced by whisker stimulation was compared between cortical surface and parenchymal tissue in the vibrissae area of somatosensory cortex in awake Tie2-GFP mice in which the vascular endothelium had genetically expressed green fluorescent protein. We observed that a mean arterial diameter during a pre-stimulus baseline state was 39 ± 7, 19 ± 1, 16 ± 4, 17 ± 4, and 14 ± 3 μm at depths of 0, 100, 200, 300, and 400 μm, respectively. The stimulation-evoked dilation induced by mechanical whisker deflection (10 Hz for 5 s) was 3.4 ± 0.8, 1.8 ± 0.8, 1.8 ± 0.9, 1.6 ± 0.9, and 1.5 ± 0.6 μm at each depth, respectively. Consequently, no significant differences were observed for the vessel dilation rate between the cortical surface and parenchymal arteries: 8.8 %, 9.9 %, 10.9 %, 9.2 %, and 10.3 % relative to their baseline diameters, respectively. These preliminary results demonstrate that the present method is useful to further investigate the quantitative relationships between the spatiotemporally varying arterial tone and the associated blood flow changes in the parenchymal microcirculation to reveal the regulatory mechanism of the cerebral blood flow.},
 pages = {419--425},
 title = {Measuring the vascular diameter of brain surface and parenchymal arteries in awake mouse.},
 volume = {789},
 year = {2013}
}