@article{oai:repo.qst.go.jp:00046888,
 author = {Tajima, Yosuke and Takuwa, Hiroyuki and Kokuryo, Daisuke and Kawaguchi, Hiroshi and Seki, Chie and Masamoto, Kazuto and Ikoma, Yoko and Taniguchi, Junko and Aoki, Ichio and Tomita, Yutaka and Suzuki, Norihiro and Kanno, Iwao and Saeki, Naokatsu and Ito, Hiroshi and 田島 洋佑 and 田桑 弘之 and 國領 大介 and 川口 拓之 and 関 千江 and 正本 和人 and 生駒 洋子 and 谷口 順子 and 青木 伊知男 and 冨田 裕 and 菅野 巖 and 伊藤 浩},
 journal = {Journal of cerebral blood flow and metabolism : official journal of the International Society of Cerebral Blood Flow and Metabolism},
 month = {May},
 note = {This study aimed to examine the cortical microvessel diameter response to hypercapnia in misery perfusion using two-photon laser scanning microscopy (TPLSM). We evaluated whether the vascular response to hypercapnia could represent the cerebrovascular reserve. Cerebral blood flow (CBF) during normocapnia and hypercapnia was measured by laser-Doppler flowmetry through cranial windows in awake C57/BL6 mice before and at 1, 7, 14, and 28 days after unilateral common carotid artery occlusion (UCCAO). Diameters of the cortical microvessels during normocapnia and hypercapnia were also measured by TPLSM. Cerebral blood flow and the vascular response to hypercapnia were decreased after UCCAO. Before UCCAO, vasodilation during hypercapnia was found primarily in arterioles (22.9%±3.5%). At 14 days after UCCAO, arterioles, capillaries, and venules were autoregulatorily dilated by 79.5%±19.7%, 57.2%±32.3%, and 32.0%±10.8%, respectively. At the same time, the diameter response to hypercapnia in arterioles was significantly decreased to 1.9%±1.5%. A significant negative correlation was observed between autoregulatory vasodilation and the diameter response to hypercapnia in arterioles. Our findings indicate that arterioles play main roles in both autoregulatory vasodilation and hypercapnic vasodilation, and that the vascular response to hypercapnia can be used to estimate the cerebrovascular reserve.Journal of Cerebral Blood Flow & Metabolism advance online publication, 21 May 2014; doi:10.1038/jcbfm.2014.91.},
 pages = {1363--1372},
 title = {Changes in cortical microvasculature during misery perfusion measured by two-photon laser scanning microscopy.},
 volume = {34},
 year = {2014}
}