{"created":"2023-05-15T14:36:21.139544+00:00","id":46804,"links":{},"metadata":{"_buckets":{"deposit":"397a5ce5-8fe9-4791-a71c-84708b1e6f39"},"_deposit":{"created_by":1,"id":"46804","owners":[1],"pid":{"revision_id":0,"type":"depid","value":"46804"},"status":"published"},"_oai":{"id":"oai:repo.qst.go.jp:00046804","sets":["1"]},"author_link":["466874","466875","466870","466872","466871","466868","466873","466869"],"item_8_biblio_info_7":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2014-05","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"5","bibliographicPageStart":"e96666","bibliographicVolumeNumber":"9","bibliographic_titles":[{"bibliographic_title":"PLOS ONE"}]}]},"item_8_description_5":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"Temperature-sensitive (ts) CHO-K1 mutant tsTM3 exhibits chromosomal instability and cell-cycle arrest in the S to G2 phases with decreased DNA synthesis at the nonpermissive temperature, 39°C. Previously, complementation tests with other mutants showed that tsTM3 harbors a genetic defect in the ubiquitin-activating enzyme Uba1. Sequence comparison of the Uba1 gene between wild-type and mutant cells in this study revealed that the mutant phenotype is caused by a G-to-A transition that yields a Met-to-Ile substitution at position 256 in hamster Uba1. The ts defects in tsTM3 were complemented by expression of the wild-type Uba1 tagged with green fluorescent protein. Expression of the Uba1 primarily in the nucleus appeared to rescue tsTM3 cells. Incubation at 39°C resulted in a decrease of nuclear Uba1 in tsTM3 cells, suggesting that loss of Uba1 in the nucleus may lead to the ts defects. Analyses with the fluorescent ubiquitination-based cell cycle indicator revealed that loss of function of Uba1 leads to failure of the ubiquitin system in the nucleus. Incubation at 39°C caused an increase in endogenous geminin in tsTM3 cells. A ts mutation of Uba1 found in tsTM3 cells appears to be a novel mutation reflecting the important roles of Uba1 in nucleus.","subitem_description_type":"Abstract"}]},"item_8_publisher_8":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"PLOS"}]},"item_8_relation_13":{"attribute_name":"PubMed番号","attribute_value_mlt":[{"subitem_relation_type_id":{"subitem_relation_type_id_text":"24805847","subitem_relation_type_select":"PMID"}}]},"item_8_relation_14":{"attribute_name":"DOI","attribute_value_mlt":[{"subitem_relation_type_id":{"subitem_relation_type_id_text":"10.1371/journal.pone.0096666","subitem_relation_type_select":"DOI"}}]},"item_8_relation_17":{"attribute_name":"関連サイト","attribute_value_mlt":[{"subitem_relation_name":[{"subitem_relation_name_text":"http://www.plosone.org/article/info%3Adoi%2F10.1371%2Fjournal.pone.0096666"}],"subitem_relation_type_id":{"subitem_relation_type_id_text":"http://www.plosone.org/article/info%3Adoi%2F10.1371%2Fjournal.pone.0096666","subitem_relation_type_select":"URI"}}]},"item_8_source_id_9":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"1932-6203","subitem_source_identifier_type":"ISSN"}]},"item_access_right":{"attribute_name":"アクセス権","attribute_value_mlt":[{"subitem_access_right":"metadata only access","subitem_access_right_uri":"http://purl.org/coar/access_right/c_14cb"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"菅谷, 公彦"}],"nameIdentifiers":[{"nameIdentifier":"466868","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"石原, よし江"}],"nameIdentifiers":[{"nameIdentifier":"466869","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"井上, 園江"}],"nameIdentifiers":[{"nameIdentifier":"466870","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"辻, 秀雄"}],"nameIdentifiers":[{"nameIdentifier":"466871","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"菅谷 公彦","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"466872","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"石原 よし江","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"466873","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"井上 園江","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"466874","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"辻 秀雄","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"466875","nameIdentifierScheme":"WEKO"}]}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"Characterization of ubiquitin-activating enzyme Uba1 in the nucleus by its mammalian temperature-sensitive mutant","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Characterization of ubiquitin-activating enzyme Uba1 in the nucleus by its mammalian temperature-sensitive mutant"}]},"item_type_id":"8","owner":"1","path":["1"],"pubdate":{"attribute_name":"公開日","attribute_value":"2014-08-20"},"publish_date":"2014-08-20","publish_status":"0","recid":"46804","relation_version_is_last":true,"title":["Characterization of ubiquitin-activating enzyme Uba1 in the nucleus by its mammalian temperature-sensitive mutant"],"weko_creator_id":"1","weko_shared_id":-1},"updated":"2023-05-15T23:47:25.483286+00:00"}