@article{oai:repo.qst.go.jp:00046686,
 author = {Iida, Shuji and Imai, Kensuke and Matsuda, Sachiko and Kokuryo, Daisuke and Aoki, Ichio and et.al and 國領 大介 and 青木 伊知男},
 issue = {6},
 journal = {Journal of Magnetic Resonance Imaging : JMRI},
 month = {Apr},
 note = {Purpose: To develop a sentinel lymph node (SN) identification
method using accurately synthesized magnetic
nanoparticles (MNPs), as an enhanced specific SN tracer
in combination with magnetic resonance imaging (MRI) in
intact rodent and SN metastasis models.
Materials and Methods: Three sizes of MNPs were originally
synthesized. We developed an experimental rat SN
model, with brachial lymph nodes (Br) as the SN and the
axillary lymph node (Ax) as the second lymph node, and
injection of MNPs via the front paw. SN detectability was
evaluated in vivo using T1-weighted MR images after
injection of the synthesized MNPs, and the amount of iron
in the Br and in the Ax was assessed using inductively
coupled plasma optical emission spectrometry.
Results: The highest ratios of the amount of iron in the
Br versus the Ax were 3.1 and 3.3, using 20-nm MNPs after
2- and 24-hour injections. The appropriate dose and
particle diameter for MRI detection was optimized, and
the SN was optimally distinguished in the normal and
metastatic rat models using MRI after a 0.4 mg/kg 20-nm
MNP injection.
Conclusion: We developed and optimized a useful S Nidentification method using MRI in rodent models.},
 pages = {1346--1355},
 title = {In Vivo Identification of Sentinel Lymph Nodes Using MRI and Size-Controlled and Monodispersed Magnetite Nanoparticles},
 volume = {38},
 year = {2013}
}