{"created":"2023-05-15T14:36:13.732949+00:00","id":46635,"links":{},"metadata":{"_buckets":{"deposit":"1af3d623-8532-44ff-86f1-3222589b31d8"},"_deposit":{"created_by":1,"id":"46635","owners":[1],"pid":{"revision_id":0,"type":"depid","value":"46635"},"status":"published"},"_oai":{"id":"oai:repo.qst.go.jp:00046635","sets":["1"]},"author_link":["464926","464929","464932","464930","464928","464931","464927","464925","464924"],"item_8_biblio_info_7":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2012-12","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"3-4","bibliographicPageEnd":"136","bibliographicPageStart":"131","bibliographicVolumeNumber":"429","bibliographic_titles":[{"bibliographic_title":"Biochemical and Biophysical Research Communications"}]}]},"item_8_description_5":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"Nonhomologous end joining (NHEJ) and homologous recombination (HR) are known as DNA double-strand break (DSB) repair pathways. It has been reported that DNA-PK, a member of PI3 kinase family, promotes NHEJ and aberrant DNA-PK causes NHEJ deficiency. However, in this study, we demonstrate that a wild-type cell line treated with DNA-PK inhibitor and a mutant cell line with dysfunctional DNA-PK showed decreased HR efficiency in fish cells (Medaka, Oryzias latipes). Previously, we reported that the radiation-sensitive mutant RIC1 strain has a defect in the Histone H2AX phosphorylation after γ-irradiation. Here, we showed that a DNA-PK inhibitor, NU7026, treatment resulted in significant reduction in the number of γH2AX foci after γ-irradiation in wild-type cells, but had no significant effect in RIC1 cells. In addition, RIC1 cells showed significantly lower levels of DNA-PK kinase activity compared with wild-type cells. We investigated NHEJ and HR efficiency after induction of DSBs. Wild-type cells treated with NU7026 and RIC1 cells showed decreased HR efficiency. These results indicated that aberrant DNA-PK causes the reduction in the number of γH2AX foci and HR efficiency in RIC1 cells. We performed phosphorylated DNA-PKcs (Thr2609) and 53BP1 focus assay after γ-irradiation. RIC1 cells showed significant reduction in the number of phosphorylated DNA-PKcs foci and no deference in the number of 53BP1 foci compared with wild-type cells. These results suggest that low level of DNA-PK activity causes aberrant DNA-PKcs autophosphorylation in RIC1 cells. It is known that 53BP1 is involved in both DNA-PK dependent and independent NHEJ. Therefore we suggest that DNA-PK independent NHEJ repair DSBs under the condition of decreased DNA-PK activity, which causes reduction of HR efficiency.","subitem_description_type":"Abstract"}]},"item_8_source_id_9":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"0006-291X","subitem_source_identifier_type":"ISSN"}]},"item_access_right":{"attribute_name":"アクセス権","attribute_value_mlt":[{"subitem_access_right":"metadata only access","subitem_access_right_uri":"http://purl.org/coar/access_right/c_14cb"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Urushihara, Yusuke"}],"nameIdentifiers":[{"nameIdentifier":"464924","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Kobayashi, Jyunya"}],"nameIdentifiers":[{"nameIdentifier":"464925","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Matsumoto, Yoshihisa"}],"nameIdentifiers":[{"nameIdentifier":"464926","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Komatsu, Kenshi"}],"nameIdentifiers":[{"nameIdentifier":"464927","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Oda, Shoji"}],"nameIdentifiers":[{"nameIdentifier":"464928","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Mitani, Hiroshi"}],"nameIdentifiers":[{"nameIdentifier":"464929","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"小林 純也","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"464930","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"松本 義久","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"464931","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"小松 賢志","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"464932","nameIdentifierScheme":"WEKO"}]}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"DNA-PK inhibition causes a low level of H2AX phosphorylation and homologous recombination repair in Medaka (Oryzias latipes) cells.","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"DNA-PK inhibition causes a low level of H2AX phosphorylation and homologous recombination repair in Medaka (Oryzias latipes) cells."}]},"item_type_id":"8","owner":"1","path":["1"],"pubdate":{"attribute_name":"公開日","attribute_value":"2013-11-26"},"publish_date":"2013-11-26","publish_status":"0","recid":"46635","relation_version_is_last":true,"title":["DNA-PK inhibition causes a low level of H2AX phosphorylation and homologous recombination repair in Medaka (Oryzias latipes) cells."],"weko_creator_id":"1","weko_shared_id":-1},"updated":"2023-05-15T23:49:25.537407+00:00"}