{"created":"2023-05-15T14:36:10.013792+00:00","id":46557,"links":{},"metadata":{"_buckets":{"deposit":"62149830-a955-4725-bb5e-f434ade0afbc"},"_deposit":{"created_by":1,"id":"46557","owners":[1],"pid":{"revision_id":0,"type":"depid","value":"46557"},"status":"published"},"_oai":{"id":"oai:repo.qst.go.jp:00046557","sets":["1"]},"author_link":["464024","464014","464021","464020","464015","464016","464025","464018","464022","464023","464019","464017"],"item_8_biblio_info_7":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2013-05","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"1","bibliographicPageEnd":"106","bibliographicPageStart":"100","bibliographicVolumeNumber":"435","bibliographic_titles":[{"bibliographic_title":"Biochemical and Biophysical Research Communications"}]}]},"item_8_description_5":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"Poly(ADP-ribose) glycohydrolase (Parg) is the main enzyme involved in poly(ADP-ribose) degradation. Here, the effects of Parg deficiency on sensitivity to low and high linear-energy-transfer (LET) radiation were investigated in mouse embryonic stem (ES) cells. Mouse Parg-/- and poly(ADP-ribose) polymerase-1 deficient (Parp-1-/-) ES cells were used and responses to low and high LET radiation were assessed by clonogenic survival and biochemical and biological analysis methods.\nParg-/-cells were more sensitive to gamma-irradiation than Parp-1-/- cells. Transient accumulation of poly(ADP-ribose) was enhanced in Parg-/-cells. Augmented levels of phosphorylated H2AX (gamma-H2AX)from early phase were observed in Parg-/- ES cells. The induction level of p53 phophorylation at ser18 was similar in wild-type and Parp-1-/- cells and apoptotic cell death process was mainly observed in the both genotypes. These results suggested that the enhanced sensitivity of Parg-/- ES cells to gamma-irradiation involved defective repair of DNA double strand breaks. The effects of Parg and Parp-1 deficiency on the ES cell response to carbon-ion irradiation (LET13 and 70 keV/um) and Fe-ion irradiation (200 keV/um) were also examined. Parg-/- cells were more sensitive to LET 70 keV/um carbon-ion irradiation than Parp-1-/- cells. Enhanced apoptotic cell death also accompanied augmented levels of gamma-H2AX in a biphasic manner peaked at 1 and 24 h. The induction level of p53 phophorylation at\nser18 was not different between wild-type and Parg-/- cells. The augmented level of poly(ADP-ribose) accumulation was noted after carbon-ion irradiation compared to gamma-irradiation even in the wild-type cells. An enhanced poly(ADP-ribose) accumulation was further observed in Parg-/- cells. Both Parg-/- cells and Parp-1-/- cells did not show sensitization to Fe-ion irradiation. Parg deficiency sensitizes mouse ES cells to a wide therapeutic range of LET radiation through the effects on DNA double strand break repair responses and enhanced cell death.","subitem_description_type":"Abstract"}]},"item_8_source_id_9":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"0006-291X","subitem_source_identifier_type":"ISSN"}]},"item_access_right":{"attribute_name":"アクセス権","attribute_value_mlt":[{"subitem_access_right":"metadata only access","subitem_access_right_uri":"http://purl.org/coar/access_right/c_14cb"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Shirai, Hidenori"}],"nameIdentifiers":[{"nameIdentifier":"464014","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Fujimori, Hiroaki"}],"nameIdentifiers":[{"nameIdentifier":"464015","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Hirai, Takahisa"}],"nameIdentifiers":[{"nameIdentifier":"464016","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Sasai, Keisuke"}],"nameIdentifiers":[{"nameIdentifier":"464017","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Okayasu, Ryuichi"}],"nameIdentifiers":[{"nameIdentifier":"464018","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Masutani, Mitsuko"}],"nameIdentifiers":[{"nameIdentifier":"464019","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"et.al"}],"nameIdentifiers":[{"nameIdentifier":"464020","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"白井 秀徳","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"464021","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"平井 崇久","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"464022","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"笹井 啓資","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"464023","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"岡安 隆一","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"464024","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"益谷 美都子","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"464025","nameIdentifierScheme":"WEKO"}]}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"Parg deficiency confers radio-sensitization through enhanced cell death in mouse ES cells exposed to various forms of ionizing radiation","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Parg deficiency confers radio-sensitization through enhanced cell death in mouse ES cells exposed to various forms of ionizing radiation"}]},"item_type_id":"8","owner":"1","path":["1"],"pubdate":{"attribute_name":"公開日","attribute_value":"2013-06-05"},"publish_date":"2013-06-05","publish_status":"0","recid":"46557","relation_version_is_last":true,"title":["Parg deficiency confers radio-sensitization through enhanced cell death in mouse ES cells exposed to various forms of ionizing radiation"],"weko_creator_id":"1","weko_shared_id":-1},"updated":"2023-05-15T23:50:20.327501+00:00"}