@article{oai:repo.qst.go.jp:00046538, author = {Nikolova, Biliana and Zhelev, Zhivko and Bakalova-Zheleva, Rumiana and Aoki, Ichio and Saga, Tsuneo and et.al and Zhelev Zhivko and バカロバ ルミアナ and 青木 伊知男 and 佐賀 恒夫}, issue = {3}, journal = {Sensors (Online Only URL:http://www.mdpi.org/sensors/)}, month = {Mar}, note = {The study aimed to clarify the role of electric pulses in combination with chemotherapy on the viability of keratinocyte cell line HaCaT, in the context of its application as a new therapeutic approach for psoriasis. The data show that electroporation of HaCaT cells in combination with rifampicin induces cytoskeleton disruption and increases permeability of cell monolayer due to cell-cell junctions interruption, visualized by fluorescent imaging of E-cadherin and actin integrity. This was accompanied with synergistic reduction of cell viability. The study proposes a new opportunity for more effective skin treatment than chemotherapy. The future application of this electrochemotherapeutic approach for combined local treatment of psoriasis may have serous benefits because of a high possibility to avoid side-effects of conventional chemotherapy.}, pages = {3625--3634}, title = {Fluorescent Imaging for Assessment of the Effect of Combined Application of Electroporation and Rifampicin on HaCaT Cells as a New Therapeutic Approach for Psoriasis}, volume = {13}, year = {2013} }