{"created":"2023-05-15T14:36:04.612037+00:00","id":46435,"links":{},"metadata":{"_buckets":{"deposit":"6a1c2a67-2b49-4504-9fab-2e0bdb23b931"},"_deposit":{"created_by":1,"id":"46435","owners":[1],"pid":{"revision_id":0,"type":"depid","value":"46435"},"status":"published"},"_oai":{"id":"oai:repo.qst.go.jp:00046435","sets":["1"]},"author_link":["462568","462571","462570","462567","462573","462575","462576","462569","462574","462572"],"item_8_biblio_info_7":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2012-09","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"22","bibliographicPageEnd":"6582","bibliographicPageStart":"6579","bibliographicVolumeNumber":"20","bibliographic_titles":[{"bibliographic_title":"Bioorganic & Medicinal Chemistry"}]}]},"item_8_description_5":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"Immuno-PET is a promising approach for improved cancer diagnosis, by taking advantage of the high specificity of antibodies. Here, we present a novel cell-free protein synthesis method for preparing a positron emitter labeled-antibody. Functional anti-human EGFRvIII single chain Fv, MR1-1, was successfully labeled with carbon-11 (half-life = 20.4 min) in 5 min (36% yield) by the direct incorporation of the clinical PET tracer, l-[11C]methionine. The product [11C]MR1-1 was easily and rapidly isolated with high radiochemical purity (>95%) from the reaction solution, by affinity purification. This method would be widely applicable to the preparation of radiolabeled antibodies for PET imaging.","subitem_description_type":"Abstract"}]},"item_8_relation_14":{"attribute_name":"DOI","attribute_value_mlt":[{"subitem_relation_type_id":{"subitem_relation_type_id_text":"doi.org/10.1016/j.bmc.2012.09.038","subitem_relation_type_select":"DOI"}}]},"item_8_source_id_9":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"0968-0896","subitem_source_identifier_type":"ISSN"}]},"item_access_right":{"attribute_name":"アクセス権","attribute_value_mlt":[{"subitem_access_right":"metadata only access","subitem_access_right_uri":"http://purl.org/coar/access_right/c_14cb"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Matsuda, Takayoshi"}],"nameIdentifiers":[{"nameIdentifier":"462567","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Furumoto, Shozo"}],"nameIdentifiers":[{"nameIdentifier":"462568","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Yokoyama, Jun"}],"nameIdentifiers":[{"nameIdentifier":"462569","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Zhang, Ming-Rong"}],"nameIdentifiers":[{"nameIdentifier":"462570","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Yanai, Kazuhiko"}],"nameIdentifiers":[{"nameIdentifier":"462571","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Iwata, Ren"}],"nameIdentifiers":[{"nameIdentifier":"462572","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Kigawa, Takanori"}],"nameIdentifiers":[{"nameIdentifier":"462573","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"古本 祥三","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"462574","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"張 明栄","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"462575","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"岩田 錬","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"462576","nameIdentifierScheme":"WEKO"}]}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"Rapid biochemical synthesis of (11)C-labeled single chain variable fragment antibody for immuno-PET by cell-free protein synthesis.","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Rapid biochemical synthesis of (11)C-labeled single chain variable fragment antibody for immuno-PET by cell-free protein synthesis."}]},"item_type_id":"8","owner":"1","path":["1"],"pubdate":{"attribute_name":"公開日","attribute_value":"2012-11-16"},"publish_date":"2012-11-16","publish_status":"0","recid":"46435","relation_version_is_last":true,"title":["Rapid biochemical synthesis of (11)C-labeled single chain variable fragment antibody for immuno-PET by cell-free protein synthesis."],"weko_creator_id":"1","weko_shared_id":-1},"updated":"2023-05-15T23:51:50.534237+00:00"}