@article{oai:repo.qst.go.jp:00046435,
 author = {Matsuda, Takayoshi and Furumoto, Shozo and Yokoyama, Jun and Zhang, Ming-Rong and Yanai, Kazuhiko and Iwata, Ren and Kigawa, Takanori and 古本 祥三 and 張 明栄 and 岩田 錬},
 issue = {22},
 journal = {Bioorganic & Medicinal Chemistry},
 month = {Sep},
 note = {Immuno-PET is a promising approach for improved cancer diagnosis, by taking advantage of the high specificity of antibodies. Here, we present a novel cell-free protein synthesis method for preparing a positron emitter labeled-antibody. Functional anti-human EGFRvIII single chain Fv, MR1-1, was successfully labeled with carbon-11 (half-life = 20.4 min) in 5 min (36% yield) by the direct incorporation of the clinical PET tracer, l-[11C]methionine. The product [11C]MR1-1 was easily and rapidly isolated with high radiochemical purity (>95%) from the reaction solution, by affinity purification. This method would be widely applicable to the preparation of radiolabeled antibodies for PET imaging.},
 pages = {6579--6582},
 title = {Rapid biochemical synthesis of (11)C-labeled single chain variable fragment antibody for immuno-PET by cell-free protein synthesis.},
 volume = {20},
 year = {2012}
}