{"created":"2023-05-15T14:36:01.770015+00:00","id":46369,"links":{},"metadata":{"_buckets":{"deposit":"0301371d-5857-44c2-9195-e79cd5b76b85"},"_deposit":{"created_by":1,"id":"46369","owners":[1],"pid":{"revision_id":0,"type":"depid","value":"46369"},"status":"published"},"_oai":{"id":"oai:repo.qst.go.jp:00046369","sets":["1"]},"author_link":["461898","461907","461901","461899","461902","461900","461905","461906","461897","461904","461903"],"item_8_biblio_info_7":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2011-08","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"6","bibliographicPageEnd":"817","bibliographicPageStart":"807","bibliographicVolumeNumber":"38","bibliographic_titles":[{"bibliographic_title":"Nuclear Medicine and Biology"}]}]},"item_8_description_5":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"Introduction: The diagnosis of infection and the ability to distinguish bacterial infection from nonbacterial inflammation by positron\nemission tomography (PET) have gained interest in recent years, but still few specific radiopharmaceuticals are available for use. In this\nstudy, we developed a new radiosynthesis method of 2-deoxy-2-[18F]fluoroacetamido-D-glucopyranose ([18F]FAG) by applying\nmicrowave irradiation and demonstrated that [18F]FAG could be a potential radiopharmaceutical to distinguish bacterial infection from\nnonbacterial inflammation.\nMethods: 1,3,4,6-Tetra-O-acetyl-2-deoxy-2-bromoacetamido-D-glucopyranose was used as precursor, and labeling was performed under\nmicrowave irradiation conditions followed by alkaline hydrolysis and high-performance liquid chromatography (HPLC) purification. In vitro\nuptake of [18F]FAG by Escherichia coli was performed. Tissue biodistribution of [18F]FAG was performed in mice. Moreover, PET imaging\nacquisition of E. coli infection and nonbacterial inflammation models was performed in rats. Tissue radiotracer-accumulated sites were\nanalyzed by hematoxylin and eosin staining and anti–E.coli immunostaining.\nResults: The radiosynthesis of [18F]FAG was achieved with microwave irradiation, and the radiochemical yield was 9.7%+-2.8% end of\nbombardment (EOB); the radiochemical purity was more than 98%, and the total synthesis time was 62 min. Compared with control group, in\nvitro uptake of [18F]FAG by E. coli was significantly decrease in inhibition group (Pb.05). Biodistribution studies in mice showed rapid\nclearance of [18F]FAG from the animal body. [18F]FAG clearly visualized the infection areas but not nonbacterial inflammation areas in PET\nstudies. Quantitative analysis revealed that the uptake of [18F]FAG into infection areas was significantly higher than that of [18F]FAG into\ninflammation areas (Pb.05). Histological analysis demonstrated the presence of bacterial cells at the sites of accumulation of [18F]FAG.\nConclusions: Using 1,3,4,6-tetra-O-acetyl-2-deoxy-2-bromoacetamido-D-glucopyranose as a precursor, the new radiosynthesis method of\n[18F]FAG was achieved in fewer steps and with a shorter synthesis time than previously reported. Furthermore, [18F]FAG was able to\ndistinguish bacterial infection from nonbacterial inflammation.","subitem_description_type":"Abstract"}]},"item_8_source_id_9":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"0969-8051","subitem_source_identifier_type":"ISSN"}]},"item_access_right":{"attribute_name":"アクセス権","attribute_value_mlt":[{"subitem_access_right":"metadata only access","subitem_access_right_uri":"http://purl.org/coar/access_right/c_14cb"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Martinez, Miguel"}],"nameIdentifiers":[{"nameIdentifier":"461897","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Kiyono, Yashushi"}],"nameIdentifiers":[{"nameIdentifier":"461898","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Noriki, Sakon"}],"nameIdentifiers":[{"nameIdentifier":"461899","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Inai, Kunihiro"}],"nameIdentifiers":[{"nameIdentifier":"461900","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Mandap, Katheryn"}],"nameIdentifiers":[{"nameIdentifier":"461901","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Mori, Tetsuya"}],"nameIdentifiers":[{"nameIdentifier":"461902","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Okazawa, Hidehiko"}],"nameIdentifiers":[{"nameIdentifier":"461903","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Fujibayashi, Yasuhisa"}],"nameIdentifiers":[{"nameIdentifier":"461904","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Ido, Tatsuo"}],"nameIdentifiers":[{"nameIdentifier":"461905","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"et.al"}],"nameIdentifiers":[{"nameIdentifier":"461906","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"藤林 康久","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"461907","nameIdentifierScheme":"WEKO"}]}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"New radiosynthesis of 2-deoxy-2-[18F]flouroacetamido-D-glucopyranose and its evaluation as a bacterial infections imaging agent","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"New radiosynthesis of 2-deoxy-2-[18F]flouroacetamido-D-glucopyranose and its evaluation as a bacterial infections imaging agent"}]},"item_type_id":"8","owner":"1","path":["1"],"pubdate":{"attribute_name":"公開日","attribute_value":"2012-07-31"},"publish_date":"2012-07-31","publish_status":"0","recid":"46369","relation_version_is_last":true,"title":["New radiosynthesis of 2-deoxy-2-[18F]flouroacetamido-D-glucopyranose and its evaluation as a bacterial infections imaging agent"],"weko_creator_id":"1","weko_shared_id":-1},"updated":"2023-05-15T23:52:39.652417+00:00"}