@article{oai:repo.qst.go.jp:00046369,
 author = {Martinez, Miguel and Kiyono, Yashushi and Noriki, Sakon and Inai, Kunihiro and Mandap, Katheryn and Mori, Tetsuya and Okazawa, Hidehiko and Fujibayashi, Yasuhisa and Ido, Tatsuo and et.al and 藤林 康久},
 issue = {6},
 journal = {Nuclear Medicine and Biology},
 month = {Aug},
 note = {Introduction: The diagnosis of infection and the ability to distinguish bacterial infection from nonbacterial inflammation by positron
emission tomography (PET) have gained interest in recent years, but still few specific radiopharmaceuticals are available for use. In this
study, we developed a new radiosynthesis method of 2-deoxy-2-[18F]fluoroacetamido-D-glucopyranose ([18F]FAG) by applying
microwave irradiation and demonstrated that [18F]FAG could be a potential radiopharmaceutical to distinguish bacterial infection from
nonbacterial inflammation.
Methods: 1,3,4,6-Tetra-O-acetyl-2-deoxy-2-bromoacetamido-D-glucopyranose was used as precursor, and labeling was performed under
microwave irradiation conditions followed by alkaline hydrolysis and high-performance liquid chromatography (HPLC) purification. In vitro
uptake of [18F]FAG by Escherichia coli was performed. Tissue biodistribution of [18F]FAG was performed in mice. Moreover, PET imaging
acquisition of E. coli infection and nonbacterial inflammation models was performed in rats. Tissue radiotracer-accumulated sites were
analyzed by hematoxylin and eosin staining and anti–E.coli immunostaining.
Results: The radiosynthesis of [18F]FAG was achieved with microwave irradiation, and the radiochemical yield was 9.7%+-2.8% end of
bombardment (EOB); the radiochemical purity was more than 98%, and the total synthesis time was 62 min. Compared with control group, in
vitro uptake of [18F]FAG by E. coli was significantly decrease in inhibition group (Pb.05). Biodistribution studies in mice showed rapid
clearance of [18F]FAG from the animal body. [18F]FAG clearly visualized the infection areas but not nonbacterial inflammation areas in PET
studies. Quantitative analysis revealed that the uptake of [18F]FAG into infection areas was significantly higher than that of [18F]FAG into
inflammation areas (Pb.05). Histological analysis demonstrated the presence of bacterial cells at the sites of accumulation of [18F]FAG.
Conclusions: Using 1,3,4,6-tetra-O-acetyl-2-deoxy-2-bromoacetamido-D-glucopyranose as a precursor, the new radiosynthesis method of
[18F]FAG was achieved in fewer steps and with a shorter synthesis time than previously reported. Furthermore, [18F]FAG was able to
distinguish bacterial infection from nonbacterial inflammation.},
 pages = {807--817},
 title = {New radiosynthesis of 2-deoxy-2-[18F]flouroacetamido-D-glucopyranose and its evaluation as a bacterial infections imaging agent},
 volume = {38},
 year = {2011}
}