{"created":"2023-05-15T14:36:01.427974+00:00","id":46361,"links":{},"metadata":{"_buckets":{"deposit":"6a2f665a-f557-498f-88d9-39f8a809082b"},"_deposit":{"created_by":1,"id":"46361","owners":[1],"pid":{"revision_id":0,"type":"depid","value":"46361"},"status":"published"},"_oai":{"id":"oai:repo.qst.go.jp:00046361","sets":["1"]},"author_link":["461825","461831","461823","461824","461826","461827","461830","461828","461829"],"item_8_biblio_info_7":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2012-06","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"4","bibliographicPageEnd":"660","bibliographicPageStart":"654","bibliographicVolumeNumber":"423","bibliographic_titles":[{"bibliographic_title":"Biochemical and Biophysical Research Communications"}]}]},"item_8_description_5":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"Although mutations and deletions in the p53 tumor suppressor gene lead to resistance to low linear energy transfer (LET) radiation, high-LET radiation efficiently induces cell lethality and apoptosis regardless of the p53 gene status in cancer cells. Recently, it has been suggested that the induction of p53-independent apoptosis takes place through the activation of Caspase-9 which results in the cleavage of Caspase-3 and poly (ADP-ribose) polymerase (PARP). This study was designed to examine if high-LET radiation depresses serine/threonine protein kinase B (PKB, also known as Akt) and Akt-related proteins. Human gingival cancer cells (Ca9–22 cells) harboring a mutated p53 (mp53) gene were irradiated with 2 Gy of X-rays or Fe-ion beams. The cellular contents of Akt-related proteins participating in cell survival signaling were analyzed with Western Blotting 1, 2, 3 and 6 h after irradiation. Cell cycle distributions after irradiation were assayed with flow cytometric analysis. Akt-related protein levels decreased when cells were irradiated with high-LET radiation. High-LET radiation increased G2/M phase arrests and suppressed the progression of the cell cycle much more efficiently when compared to low-LET radiation. These results suggest that high-LET radiation enhances apoptosis through the activation of Caspase-3 and Caspase-9, and suppresses cell growth by suppressing Akt-related signaling, even in mp53 bearing cancer cells.","subitem_description_type":"Abstract"}]},"item_8_relation_14":{"attribute_name":"DOI","attribute_value_mlt":[{"subitem_relation_type_id":{"subitem_relation_type_id_text":"10.1016/j.bbrc.2012.06.004","subitem_relation_type_select":"DOI"}}]},"item_8_source_id_9":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"0006-291X","subitem_source_identifier_type":"ISSN"}]},"item_access_right":{"attribute_name":"アクセス権","attribute_value_mlt":[{"subitem_access_right":"metadata only access","subitem_access_right_uri":"http://purl.org/coar/access_right/c_14cb"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Nakagawa, Yosuke"}],"nameIdentifiers":[{"nameIdentifier":"461823","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Takahashi, Akihisa"}],"nameIdentifiers":[{"nameIdentifier":"461824","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Furusawa, Yoshiya"}],"nameIdentifiers":[{"nameIdentifier":"461825","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Ohnishi, Takeo"}],"nameIdentifiers":[{"nameIdentifier":"461826","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"et.al"}],"nameIdentifiers":[{"nameIdentifier":"461827","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"仲川 洋介","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"461828","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"高橋 昭久","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"461829","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"古澤 佳也","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"461830","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"大西 武雄","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"461831","nameIdentifierScheme":"WEKO"}]}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"Depression of p53-independent Akt survival signals in human oral cancer cells bearing mutated p53 gene after exposure to high-LET radiation","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Depression of p53-independent Akt survival signals in human oral cancer cells bearing mutated p53 gene after exposure to high-LET radiation"}]},"item_type_id":"8","owner":"1","path":["1"],"pubdate":{"attribute_name":"公開日","attribute_value":"2012-07-18"},"publish_date":"2012-07-18","publish_status":"0","recid":"46361","relation_version_is_last":true,"title":["Depression of p53-independent Akt survival signals in human oral cancer cells bearing mutated p53 gene after exposure to high-LET radiation"],"weko_creator_id":"1","weko_shared_id":-1},"updated":"2023-05-15T23:52:45.197332+00:00"}